(Jason) Lee 002213-065 Internal Assessment – Investigating the Relationship between Concentration of Sodium Chloride and the Rate of Reaction of Enzyme Amylase Research Question: How will changing the percentage of sodium chloride concentration affect the rate of reaction of enzyme amylase‚ measured using the absorbance of starch and iodine with a spectrophotometer. Introduction: Amylase is an enzyme that is involved in the human digestive process. Found in both the human pancreas and the
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Title of Lab: Investigating Enzymatic Activity in Sweet Potato‚ Irish Potato Extract and Milk. Aims: 1. To determine the effect of ascorbic acid on Polyphenol Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the following substrates: Caffeic Acid‚ Catecol‚ Guaicol‚ Pyragallol and Tyrosine. 3. To determine the effect of ascorbic acid on Peroxidase. 4. To determine the level of specificity of Peroxidase using the following substrates: Caffeic Acid‚ Catecol‚ Guaicol‚ Pyragallol
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There are many reasons why enzymes have such a high specificity. The first variable is an enzyme’s primary structure. A primary structure is just a combination of amino acids. There are twenty different amino acids that the primary structure can be created from. Every enzyme has a different order that the acids are placed in and each one has a different number or amino acids. The slightest change in this structure can affect a protein’s conformation and function. The secondary structure is a regular
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Enzymes are catalytic proteins that speed up chemical reactions by lowering the activation energy of the reaction. our body cells depend highly on enzymes to complete many important functions‚ such as digestion and metabolism. Enzymes are selective‚ in which each enzyme can only speed up a specific reaction. There are molecules that enzymes work with called substrates. substrate bind to an area of an enzyme called active site. There is specificity between the enzyme and substrate that react with
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nucleotides joined by covalent bonds - The sequence of bases makes up the genetic code - These bases are ’read’ in groups of three - These triplets are used to send instructions in the cell: to switch genes on and off‚ to make proteins and enzymes Unit 3 Page 1 enzymes DNA is a double strand of polynucleotides The sugar-phosphate backbone is on the outside‚ the bases are on the inside The strand is held together by hydrogen bonds between the bases A only pairs with T. G only pairs with C. ○ This
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Patrick McCrystal Enzymes: Natural Catalysts Enzymes are catalytic proteins‚ meaning they speed up chemical reactions without beingused up or altered permanently in the process. Although various enzymes use different methods‚all accomplish catalysis by lowering the activation energy for the reaction‚ thus allowing it tooccur more easily. Enzymes have very specific shapes (conformations). Part of the conformationis the active site of the enzyme‚ where the actual catalysis occurs. The specific molecule
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Enzymes An enzyme is a protein used to speed up the rate of a chemical reaction. Because they regulate the rate of chemical reactions‚ they are also called catalysts. There are many‚ many different types of enzymes‚ because for each chemical reaction that occurs‚ an enzyme specific to that reaction must be made. To act on a substrate‚ an enzyme must contain an active site. The active site is the area on the enzyme that allows the substrate and enzyme to fit together. The amino acids that are present
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Lecture 3: Enzyme kinetics Tue 17 Jan 2006 with the collaboration of Luna De Ferrari 1 Images from: D. L. Nelson‚ Lehninger Principles of Biochemistry‚ IV Edition‚ W. H. Freeman ed. A. Cornish-Bowden Fundamentals of Enzyme Kinetics‚ Portland Press‚ 2004 A. Cornish-Bowden Enzyme Kinetics‚ IRL Press‚ 1988 Computational Systems Biology Summary: • • • • • • 2 Simple enzyme kinetics Steady-state rate equations Reactions of two substrates Inhibition of enzyme activity pH dependence
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Catalase (Enzymes) Abstract In this laboratory exercise‚ studies of enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the
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Enzymes What Are Enzymes? Substances that speed up chemical reactions are called catalysts. Organic catalysts are called enzymes. Enzymes are specific for one particular reaction or group of related reactions. Many reactions cannot occur without the correct enzyme present. They are often named by adding "ase" to the name of the substrate. Example: Dehydrogenases are enzymes that remove hydrogen. Induced-fit Theory The shape of the enzyme must match the shape of the substrate
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