LAB: Substrate Concentration Affecting the Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because
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AIM The aim of this investigation is to explore the effect of different concentrations of bile salts on the time taken for the lipase enzyme to break down fat. BILE Bile is a brownish bitter alkaline fluid produced by the liver and made by the hepatocytes from water‚ bile salts‚ bile pigments cholesterol and phospholipids and stored in the gall bladder. Bile is directly connected with digestion. It is released sporadically into the small intestine (duodenum) which is part of the gut in order
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Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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THE EFFECT OF CATALASE ON HYDROGEN PEROXIDE The aim of the experiments is to see if increasing the surface area of the enzyme Catalase‚ affects the relative activity of the substrate Hydrogen peroxide. Then to observe and measure the effect the Catalase has on the chemical breakdown of the hydrogen peroxide. My theory is if you keep increasing the surface area of Catalase‚ the more active sites are available to join with the substrate causing an increase in the breakdown of the hydrogen peroxide
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VARYING EFFECTS OF ENZYME CONCENTRATION ON REACTION RATES OF MALATE DEHYDROGENASE CELL BIOLOGY 13 NOVEMBER 2007 Enzymes are biological catalysts. They are proteins that speed up reactions with low concentrations. These enzyme proteins are made up of linkages of amino acids. The links coil‚ and coil again forming a tertiary structure. This structure has a groove in it called an active site. The active site is
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Introduction: The purpose of this experiment is to measure the effects of changes in temperatures and pH on enzyme activity in skeletal muscle‚ particularly the activity of lactate dehydrogenase (LDH). LDH is a glycolytic enzyme which converts pyruvate to lactate in the following equation: LDH Pyruvate+ NADH ------------ Lactate + NAD The reaction above can move in both directions‚
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Temperature and Enzyme Activity Aim: To investigate the effect of temperature on enzyme activity Hypothesis: As the temperature deviates from 40°C the activity will lower Equipment: – Chemicals: – Milk – Junket tablets – Hot water – Ice – Test tubes – Stopwatch – Measuring cylinder Risk Assessment: |Hazard |Risk |Prevention | |Hot Water
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report) Activity: Name: Instructor: Date: Enzyme Activity cheryl yelton November 30‚ 2014 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose)
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Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
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3rd-minute mark‚ so relatively fast before plateauing at 25 degrees. But the 3% took almost the whole 7 minutes to finish rising in temperature. So essentially‚ the higher the concentration of hydrogen peroxide‚ the faster the reaction will take place. Furthermore‚ the foam (water and oxygen produced by the potatoes catalase reacting with hydrogen peroxide) was a kind of indicator of how the reaction was going. It almost indirectly related to the temperature increase‚ because every time the temperature
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