Temperature affecting enzyme activity Aim: To see how temperature can affect enzyme activity Prediction:-I think the one that will work best will be the rennin placed in milk at 37oC My independent variable will be the temperature My dependant variable will be how thick the milk becomes The equipment I need is milk‚ 5 test tubes‚ rennin‚ water bath‚ boiled rennin‚ a glass rod and a stopwatch Introduction: Rennin is an enzyme found in the stomach‚ its function is to solidify milk. We
Premium Temperature Enzyme Gas
How is pH affecting catalase activity? Hypothesis The catalase activity is assumed to be the most active in pH7. The higher or lower concentration away from the optimum pH of catalase‚ the slower the rate of activity is. Data Collection & Processing I collected the results of pH4‚ pH7‚ pH10 and pH13 after 2 minutes‚ and I repeated the experiment 3 times. Below is a table to show the results‚ their averages and standard deviations: | 1st time (sec) | 2nd time (sec) | 3rd time (sec)
Free PH Buffer solution Standard deviation
Enzymes are catalysts that increase the rate of chemical reactions organisms‚ allowing cells to break or build things instantly. The structure of an enzyme is essential to its function. Enzymes are proteins‚ made up of 100-1000 amino acids bonded together in chains. These chains are folded/coiled into a unique 3-D structure that allows them to bind to a reactant‚ called a substrate at an active site. Enzymes are flexible‚ and therefore can change it’s shape to better accommodate its substrate; this
Premium Enzyme Chemical reaction Catalysis
ABSTRACT Enzymes are biological catalysts; they cause reactions to happen that would not normally occur due to the activation energy that would be required. They bring together substrates and cause chemical reactions that are essential for life. Without enzymes life processes‚ and life in of itself‚ would not be possible. Enzymes are also special because very little of the actual enzyme is actually used up in the reaction. In this lab two different factors‚ temperature and pH‚ were tested to see
Premium Enzyme PH
In this laboratory exercise we studied enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment
Premium Oxygen Enzyme Catalase
Investigating the Effect of Enzyme Concentration on Pectinase in the Production of Fruit Juice In this practical‚ I shall demonstrate the relationship between the concentration of pectinase and the amount of fruit juice produced accordingly in proportion. Apparatus: Apples Pectinase solution 10 x filter paper Knife or kitchen mincer Glass rod Water bath X 1 250 ml beakers X 2 Wash bottle of distilled water 100 cm^3 measuring cylinders In the case
Premium Enzyme Null hypothesis Juice
Abstract The purpose of this lab was to test if ethanol affects the reaction involving hydrogen peroxide and catalase. Tests were performed by putting chicken liver‚ ethanol solution (diluted ethanol solution for other trials) and hydrogen peroxide in a test tube with a side arm‚ and having a rubber tube lead the oxygen gas into a gas collection tube. Results from the tests showed a negative correlation‚ this means that the more diluted the solution of 95% ethanol was‚ the less oxygen gas collected
Premium Oxygen Hydrogen peroxide Enzyme
4.3 (b) To investigate the effect of pH on the rate of catalase activity Teacher Notes Apparatus required per class group of 24 students Chopping board 12 Knife 12 Weigh boats 12 100 ml Graduated cylinders 36 Boiling tube 36 Syringe 12 Electronic balance 3 Dropper 12 Timer 12 Labels 36 Thermometer 12 Water bath 3 Enzyme source eg. Radishes/celery Hydrogen peroxide Range of buffer solutions pH paper Washing up liquid Disposable gloves The apparatus
Free PH Buffer solution
Effects of Enzyme Catalysis of H2O2 by Catalase Report by: Timmy Lin (#269164729) October 17‚ 2011 Mr. Rienzi AP Biology Problem: Measuring the effects of Catalase enzymes on hydrogen peroxide decomposition. Measuring the rate of the reaction when hydrogen peroxide and Catalase are mixed at the same ratio for different time (10‚ 20 30 60 120 180 360 seconds). Background: Enzymes are biological catalysts that carry out cellular metabolic processes with the ability to enhance the rate
Premium Oxygen Enzyme Hydrogen peroxide
In this lab we tested the effect of temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance‚ which gave us a lower
Premium Chemical reaction Enzyme Enzyme