Enzyme kinetics (catalase/kmno4) Enzyme catalysis Farah Mohamed galal 22-3014 t09 Introduction: E + S → ES → E + P Enzymes are proteins which act as a catalyst in biochemical reactions(affect the rate of achemical reaction). The substrate binds to the active site of the enzyme. Any deformation of the active site will affect the activity of the enzyme‚ these are some ways that enzyme action may be affected because of them: 1- Salt concentration: If it is close to zero or very high
Premium Catalysis Enzyme Catalase
Background Information Part 1 In the first part of the enzyme lab‚ we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together‚ we observed and saw a change in the color of the substance. The darker and more brown the substance got‚ the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased
Premium Enzyme PH
EFFECT OF BIODEGRADABLE SM PLASTIC BAG IN DIFFERENT SUBSTRATE ABSTRACT The purpose of this Investigatory project is to determine the effect of different substrates on the biodegradability of SM plastic bags. The materials needed are: 6‚ 1-litre glass jars; 6‚ 7”x9” aluminums trays; 12 pcs. 3”x4” SM plastic bags; 500g loam soil; 500g compost soil; 500mL seawater; 500mL tap water; Analytical balance; Graduated cylinder; and Weighing scale. The procedure involved cutting the SM plastics bags
Premium Polymer Plastic
For Activity A‚ we first tested enzyme activity. First‚ we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then‚ we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that‚ we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that‚ we placed the test tube filled with catalase in a
Premium Catalase Titration Water
Enzymes are a major role in the process of accelerating chemical reactions. Environmental factors affect the enzymes‚ These environmental factors include ph levels and body temperature. While both of these environmental factors affect the enzyme‚ body temperature affects the enzymes the most and can result in death. Enzymes are affected by high body temperature. Enzymes are highly specific and they work at certain temperature levels depending if it’s hot or cold. However‚ when the
Premium Carbon dioxide Nutrition Climate change
The Effect of Enzymes on Apple Juice Production Biology Period 6 Background Info The purpose of this experiment was to determine which enzyme or the combination of the two enzymes makes the most apple juice from applesauce. We did his by‚ mixing 10 drops of an enzyme (cellulose/pectinase/both) into a few tablespoons of applesauce. We then left the rest to nature‚ and watched the liquid funnel out for 10 minutes. Lastly‚ we recorded our data. The cell wall is a complicated
Premium Enzyme Cell wall Cellulose
Enzymes (pron.: /ˈɛnzaɪmz/) are large biological molecules responsible for the thousands of chemical interconversions that sustain life.[1][2] They are highly selective catalysts‚ greatly accelerating both the rate and specificity of metabolic reactions‚ from the digestion of food to the synthesis of DNA. Most enzymes are proteins‚ although some catalytic RNA molecules have been identified. Enzymes adopt a specific three-dimensional structure‚ and may employ organic (e.g. biotin) and inorganic (e
Premium Enzyme
Title of Practical: The Effect of Concentration on Reaction Rate Aim: To use a simple reaction between sodium thiosulphate and hydrochloric acid to discover concentration this determines how fast chemical reactions occur. Independent Variable: Concentration of hydrochloric acid (%) Dependent Variable: Time taken for chemical reaction to take place (sec) Hypothesis: My prediction is that the increased concentration of the thiosulfate will in turn lead to an increase in the rate of reaction
Premium Chemical reaction Reaction rate Chemistry
An investigation into the effects of temperature on enzyme action An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems‚ for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate
Premium Temperature Chemical reaction Chemistry
Enzymes are catalytic proteins that selectively speed up chemical reactions without by consumed by the reaction itself (1). Enzyme activity is significantly affected by factors such as temperature‚ substrate concentration‚ enzyme concentration and pH. Enzymes are highly specific and only catalyse one specific chemical reaction‚ speeding up the reaction by lowering the activation energy‚ the energy required to start a chemical reaction (2). At high temperatures and with various mechanical forces or
Premium Enzyme Chemical reaction Catalysis