Hypothesis The optimum temperatures of Alcalase and Savinase will be different. Above and below their optimum temperatures activity will decrease. Biological explanation This investigation is designed to look at the effect of temperature on the activity of the proteases Alcalase and Savinase. By the end of it I hope to know the optimum temperature of both proteases. The substrate I am going to use during the experiments is the protein gelatin‚ which is a translucent‚ colourless‚ brittle solid substance
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The Effect of Concentration Gradient on Osmosis Abstract: Osmosis is the passive movement of water from an area of higher concentration to an area of lower concentration‚ usually across a membrane (Thorpe 2013). Tonicity is the ability of a solution surrounding a cell to gain or lose water (Reece 2011). There are many factors that affect the rate of osmosis. These include temperature‚ surface area‚ difference in water potential‚ pressure‚ light and dark and most importantly what we will be talking
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from 0.93-1.04 grams from the solutions containing AgNO3 (Table 1). Graph one depicts the correlation of percent change in weight for each sucrose concentration with AgNO3 added. The initial length of the potato cylinders were 2cm as depicted in table two. Graph two depicts the correlation of percent change in length in different sucrose concentrations. The initial weights for the solutions lacking AgNO3 ranged from 1.0-1.7grams (Table 3). Graph three displays the correlation for the percent change
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Effects on Survivors of Concentration Camps Many people during the 1940s were put into concentration camps‚ and after getting out‚ if they did get out‚ suffered many problems. The stress and torment these people went through was pure murder (Nelken). The humans put into these concentration camps were from many different races‚ and also included the mentally retarded‚ severely handicapped‚ insane‚ and very sick people (Austin). Survivors of concentration camps faced psychological‚ spiritual‚ physical
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There are approximately 40‚000 enzymes living in one human cell‚ each responsible for a chemical reaction. Enzymes are complex 3D protein molecules created by amino acids‚ forming a unique sequence that produces hydrogen bonds‚ eventually formulating an enzyme within plants and animals (Boyle & Senior‚ 2002). Working alongside other molecules‚ they uphold a stable reaction system. The function of an enzyme is to aid and increase chemical reactions and organise metabolism‚ while maintaining homeostasis
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Enzyme Catalysis Lab Problem: Before the lab‚ one should understand: • The general functions and activities of enzymes; • The relationship between the structure and function of enzymes • The concept of initial reaction rates of enzymes; • How the concept of free energy relates to enzyme activity; • That change in temperature‚ pH‚ enzyme concentration‚ and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and • Catalyst‚ catalysis‚ and catalase
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Aim: The aim of the experiment is to test the effect temperature has on the activity of the enzyme rennin. Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC‚ which is the body temperature‚ where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working. Introduction:Enzymes are
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Abstract Enzyme catalysis was observed in order to analyze how changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration affected an enzyme-catalyzed reaction. This experiment analyzed the rate of enzyme-catalyzed reactions and observed the correlation between catalase activity and products formed. It was found out that the rate of an enzyme-catalyzed reaction starts off rapidly‚ decreases‚ and levels off or completely stops‚ and can be further affected by environmental factors
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RESULTS AND DISCUSSION REPORT—EXPERIMENT 3 (CHEMICAL KINETICS) CALCULATIONS Effect of Concentration on Reaction Rate [S2O32-]initand [H+]init for each run‚ knowing the original concentrations and volumes of [S2O32-]‚ [H+]‚ and water used. [S2O32-]init= __(M[S2O32-])(V[S2O32-])__ [H+]init= _____(M[H+])(V[H+])____ V[S2O32-]+V[H+]+V[water] V[H+]+V[S2O32-]+V[water] Run 1 [S2O32-]init= (0.15 M)(10 mL) (10+3+2)mL = 0.1 M [H+]init= (3 M)(2
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The effect of time on enzyme reaction. Abstract: In this lab investigation we will observe how the amount of hydrogen peroxide is affected by catalase over time. The enzyme was added to 10 mL’s of hydrogen peroxide and observed over time to determine the relation between time and enzyme activity. The hypothesis stated that as time increased substrate would decrease. Therefore I predicted that at 60 seconds‚ there would be the least amount of H2O2. The enzyme activity mirrored my predictions
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