Lab activity 1: Effect of temperature‚ concentration‚ and pressure on equilibrium Introduction Our ongoing discussion has been on systems at dynamic equilibrium: for a reversible reaction‚ the rate of the forward reaction is equal to the rate of the reverse reaction. What happens if equilibrium is disturbed? In this lab activity‚ we are going to examine the effect of changing reaction conditions on the position of equilibrium. Part I: Effect of temperature We will consider the equilibrium
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Sang Kim Enzyme Catalyst Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A‚ B‚ C‚ and D. In activity A‚ the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction‚ to study the characteristics of an enzyme mediated reaction‚ and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H 2O2 present
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Properties of Enzymes and Competitive Inhibitors. Abstract: Properties of enzymes were found in this experiment and some other factors‚ which affect enzyme activity. Enzymes are catalyst; they catalyze very specific reactions. Results relating to the active site of specific enzymes played a big role while performing this experiment. The purpose of this experiment was to fin how inhibitors affect enzyme’s activity by competing for the active site against substrates. Introduction: Cells
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Enzymatic Activity of Salivary Amylase Abstract: Salivary amylase is an enzyme that can digest starch molecules and break them down to sugar molecules. In this experiment‚ the enzymatic activity and specificity of salivary amylase was examined depending on the changes in pH and temperature. In the first part of the experiment‚ the effect of temperature was determined‚ using constant temperature bath (4‚ room temp‚ 37‚ 50‚ 60‚ and 70°C). Having the room temp and 50°C as the highest and 37°C as infinite
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Effects of Human Activities on the Lake Ecosystem I. Introduction On the first exercise of the Environmental Biology class‚ the students have learned what an ecosystem is and what its significance to the survival of the living and non-living are through analysis of forest and agro-ecosystems. The ecosystem as the basic unit for ecological study (Evans‚ 1956) consists of the complex of interacting organisms inhabiting a region with all the non-living physical factors that make up their environment
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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The experimental samples for the pH concentration experiment were put together by using a 10ml-graduated cylinder to obtain 4ml of each pH buffer to insert into cuvettes‚ a micropipette was then used to obtain 0.5ml of catechol and 0.5ml of the catechol oxidase. The pH buffer was made first to avoid any denaturation of the catechol oxidase. Our positive control for this experiment was pH 7 because that is the pH level of most cell membranes in the cytoplasm (Whitson‚ 2016.) Our negative controls
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Six Factors Affecting the Performance of an Enzyme Enzymes are considered catalysts; substances that increase the rates of reactions. Enzymes are responsible for thousands of metabolic processes that involve the sustainment of life‚ one common one is simple food digestion. Without enzymes‚ digestion would occur too slowly for life to continue. Enzymes maintain a protein structure consisting of one or possibly more than one polypeptide chains of defined primary structure‚ and take up a characteristic
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Concentration Camps in Vienna The Auschwitz concentration camp complex was the largest of its kind established by the Nazi regime. It included three main camps‚ all of which deployed incarcerated prisoners at forced labor. One of them also functioned for an extended period as a killing center. The camps were located approximately 37 miles west of Krakow‚ near the prewar German-Polish border in Upper Silesia‚ an area that Nazi Germany annexed in 1939 after invading and conquering Poland. The SS authorities
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3.8.3. Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature
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