Core Practical: Enzyme concentrations and enzyme activity. Introduction In this experiment I shall investigate how the enzyme concentration can affect the initial rate of reaction. I will measure the effect of the enzyme in 5 different concentrations against the controlled variable of the reactant. The enzyme which will be used is different concentrations of potato and the reactant used will be Hydrogen Peroxide. Hydrogen Peroxide which will be the buffer solution is a PH of 7.2. My hypothesis
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Abstract The catalysis of enzymes within our bodies is essential for human survival and when this ability is impaired by the presence of Hydrogen ions‚ our cells cannot function properly. This experiment was conducted to determine if the reaction rate changes in response to a variation of acidic‚ neutral‚ and basic solutions. The experimental results indicated that the basic/high pH solution has a faster rate of reaction in the solution. Introduction Enzymes are proteins that catalyze and
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The effect on rate of an enzyme catalysed reaction by different objectives which include effect of substrate‚ temperature‚ ph and effect of a competitive inhibitor phosphate ions. This is determined by the reaction of hydrolysis by p-nitrophenylphosphate (PNP) as a substrate by the enzyme phosphatase. Abstract The hydrolysis of p-nitrophenyl phosphate has been studied in human red blood cells. To see if hydrolysis was related to the functioning of the sodium pump. Acid phosphatase catalysis’s
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Reaction of Enzyme Amylase Research Question: How will changing the percentage of sodium chloride concentration affect the rate of reaction of enzyme amylase‚ measured using the absorbance of starch and iodine with a spectrophotometer. Introduction: Amylase is an enzyme that is involved in the human digestive process. Found in both the human pancreas and the human saliva‚ amylase breaks down starch into sugar so that large molecules can be easily digested1. Like all enzymes‚ amylase must be kept
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Science investigation Aim: To try to see if the temperature affects the rate in which Amylase breaks down starch into maltose. In this reaction starch is the substrate and maltose is the product. Amylase is an enzyme‚ Enzymes‚ also called catalysts‚ are in living things and there are thousand of them. Enzymes break down food by the active site on the Enzyme forming a chemical bond with a substrate and then water attacks the substrate until it is hydrolysed (split in 2). Equipment: Boiling
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carefully‚ as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful‚ ensuring they don’t come into contact with our eyes‚ skin or clothes. Catalyse is an enzyme found in all living cells. It makes Hydrogen Peroxide decompose into water and Oxygen. We will be measuring the amount of Oxygen released from the Hydrogen Peroxide. In
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Aim To investigate the effect of different temperatures on the activity of rennin in milk Introduction Enzymes are globular protein‚ responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon‚ hydrogen‚ oxygen and nitrogen (Gunsch‚ 2012). The role of enzyme is to act as catalysts‚ substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done
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CHAPTER 4: ENZYMES Enzymes are biological catalysts. There are about 40‚000 different enzymes in human cells‚ each controlling a different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures. They were discovered in fermenting yeast in 1900 by Buchner‚ and the name enzyme means "in yeast". As well as catalysing all the metabolic reactions of cells (such as respiration
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Enzymes are catalytic proteins that selectively speed up chemical reactions without by consumed by the reaction itself (1). Enzyme activity is significantly affected by factors such as temperature‚ substrate concentration‚ enzyme concentration and pH. Enzymes are highly specific and only catalyse one specific chemical reaction‚ speeding up the reaction by lowering the activation energy‚ the energy required to start a chemical reaction (2). At high temperatures and with various mechanical forces or
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Investigating the effect of temperature on plant cell membranes The purpose of this activity is: • to practise experimental and investigative skills • to investigate the effect of temperature on cell membrane structures Procedure SAFETY: Always carry scalpels clasped to a tile and with the tip pointing away from you. Beetroot cells contain pigments called betalains that give the tissue its dark purple-red colour. The pigment is contained in the cell vacuole. Investigation
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