The effect of increasing substrate concentration on rate of an enzyme reaction. Enzymes are biological catalysts that lower a reactions activation energy making possible many of the reactions needed for life to exist. Enzymes have a high specificity which have been explained by many theories such as Fischer’s lock and key. Currently the most widely accepted theory is the induced fit hypothesis proposed by Koshland in 1958. This hypothesis solves some of the problems with the Lock and key theory
Premium Enzyme Catalysis Protein
ENZYMES LABORATORY REPORT Introduction The utilization of any complex molecule for energy by an organism is dependent on a process called hydrolysis. Hydrolysis breaks complex molecules into simpler molecules using water. Similarly‚ the process that is the reverse of this is called dehydration synthesis‚ which removes water from simpler molecules. However‚ because hydrolysis occurs very slowly‚ living organisms use biochemical’s called enzymes to speed up the reaction. In this lab exercise
Premium Enzyme
Experiment 20: pH Titration: Phosphoric Acid in Cola Drinks Post-lab Assignment or Report The post-lab report for this experiment is due at the beginning of the following lab period. Student notes for the lab will be available on the lab T-Square site. Learning Objectives Students will be able to... • Use a known mass of solid acid to determine an unknown concentration of a basic solution (this process is called “standardization”). • Execute a titration using good‚ reliable technique.
Premium Acid PH Titration
8 1 EXPERIMENTAL DATA Table 1: Coordinate of Pressure Tapping Tapping No. 1 2 3 4 5 6 7 8 9 10 11 Note: Table 2: Pressure Readings Manometer inclination: Pressure Readings Pitot Pressure Static Pressure Atmospheric Pressure Atmospheric Temperature Stall angle: At the end of the experiment 474 mm 497 mm 500 mm 29°C (mm) 0.0 2.5 5.0 10 20 30 40 50 60 70 80 (mm) 0.000 3.268 4.443 5.853 7.172 7.502 7.254 6.617 5.704 4.580 3.279 0 0.025 0.049 0.098 0.197 0.295
Premium Fluid dynamics Aerodynamics Lift
Question 1 Critique Dr. Honeydew’s report. Specifically‚ discuss what is missing and how the missing information should have been presented. First the PH measurements‚ Dr. honey dew does not explain how the PH device was calibrated or if the blood sample was added or mixed with anything prior to the measuring of the pH (like water‚ etc.). In regards to the absorption spectroscopy‚ the method by which the concentration was obtained is questionable. Was the concentration known before the experiment
Premium PH
Aim: To investigate the effect of hydrogen peroxide concentration on enzyme catalase Indroduction: Enzymes are proteins. They function as biological catalysts. They lower the energy barrier of a reaction so that the reaction can take place at body temperature. Also‚ they can speed up Metabolic reactions without being changed or used up. During a reaction‚ an enzyme molecule combines temporarily with the substrate. When the reaction is complete‚ the enzyme molecules returns to its original dorm
Premium Enzyme Oxygen Catalase
Lab Report: Purpose: The Objective of this lab was to learn how to measure the pH (or acidity) of commonly known fluids‚ using the correct tools and procedures. To then use that data to document the changes noticed when mixing those same fluids and changing their respective pH levels. Materials: In order to conduct this experiment several pieces of equipment and other materials were needed. The first item was a graduated cylinder‚ which was used in order to measure out the precise
Premium PH
Introductory Biology 1 Biology 1003 Fall Term 2011 Lab Number: 3 Title: Cell Energetics: Enzyme Role in Biological Reactions Name: Brandon Moore Student Number: 100819124 Lab day and time: Wednesday pm Date: Wednesday November 23‚ 2011 Introduction Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology
Premium Chemical reaction Enzyme
certain environmental factors affect the enzyme activity rate. For the first experiment‚ where we tested the increase in concentration of enzyme with the substrate‚ we found that higher concentration of enzyme increases the rate of reaction of the enzyme. This is because more enzyme molecules are present‚ which allow more substrate molecules to get into the active sites of the enzyme (Sattler W& Esterbauer H). When calculating the absorbance of different enzyme concentration‚ it was noticeable that
Premium Enzyme Chemical reaction Chemistry
Factors Affecting Enzymes The activity of an Enzyme is affected by its environmental conditions. Changing these alter the rate of reaction caused by the enzyme. In nature‚ organisms adjust the conditions of their enzymes to produce an Optimum rate of reaction‚ where necessary‚ or they may have enzymes which adapted to function well in extreme conditions where they live.Increasing temperature increases the Kinetic Energy. In a fluid‚ this means that there are more random collisions between molecules
Premium Enzyme PH