Determination of the Effect of pH on Amylase Activity Grace Chung Abstract: Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes‚ including amylase‚ function best at a certain optimal pH. Therefore‚ in this experiment‚ the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to that of
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Enzymatic Activity of Salivary Amylase Ong‚ Janela Rose I.; Paguia‚ Maria Tricia C.; Placente‚ Dax Daven A.; Posadas‚ Grace Catherine A. 3Bio3-Group 8 Department of Biological Sciences‚ College of Science University of Santo Tomas‚ España‚ Manila 1008 Abstract This experiment aims to examine the enzymatic activity and specificity of salivary amylase depending on the changes in pH and temperature; and determine the optimum temperature and pH of the amylase. EXPERIMENTAL In
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Title: Enzyme Activity Aim: To investigate the activity of the enzyme catalase in liver and potatoes‚ and to investigate the effect of temperature‚ surface area‚ pH and certain chemicals on the activity of catalase. Equipment: Dilute hydrochloric acid solution x 1ml 10 volume hydrogen peroxide x 100ml Copper sulphate solution x 1ml Aluminum nitrate solution x 1ml Zinc nitrate solution x 1ml dilute NaOH solution x 1ml Mortar and pestle x 1 25ml Beaker x 1 Hot plate x1
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The Effect of Increasing Temperatures on the Enzyme Catalase Enzymes are proteins that help speed up chemical reactions inside your body and without enzymes‚ chemicals reactions in cells would be incredibly slow to a point where no activity at all takes place (Brawo press Inc‚ 2017). Enzymes speed up the chemical reactions by lowering the activations energy and they do this by binding substrates together in the correct orientation to react (Ernest Z‚ 2014). They are vital for life and serve a
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nucleotides joined by covalent bonds - The sequence of bases makes up the genetic code - These bases are ’read’ in groups of three - These triplets are used to send instructions in the cell: to switch genes on and off‚ to make proteins and enzymes Unit 3 Page 1 enzymes DNA is a double strand of polynucleotides The sugar-phosphate backbone is on the outside‚ the bases are on the inside The strand is held together by hydrogen bonds between the bases A only pairs with T. G only pairs with C. ○ This
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The Effects on Enzymes By Bailey Rose The Effects on Enzymes Bailey Rose 10/31/2011 Abstract In this lab exercise‚ the study of enzyme catalase‚ we viewed the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment. Our class performed
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Abstract: This lab tests how temperature and pH affect how enzymes will function. The lab showed that temperature will denature an enzyme when past its optimal working temperature and won’t denature in cold temperatures‚ but have slowed molecular activity. pH will also have an affect on an enzymes efficiency‚ when out of optimal pH the enzyme will not function as it is supposed to and if to far out of the optimal pH the enzyme will change shape and no longer work. Enzymes also showed to be reusable
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are amylases? Amylases are enzymes that break down starch or glycogen. Amylases are produced by a variety of living organisms‚ ranging from bacteria to plants and humans. Bacteria and fungi secrete amylases to the outside of their cells to carry out extracellular digestion. When they have broken down the insoluble starch‚ the soluble end products such as (glucose or maltose) are absorbed into their cells. Amylases are classified based on how they break down starch molecules i. α-amylase (alpha-amylase)
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curve on my graph. However‚ I believe due to human error the results were incorrect between temperatures of 60Ëšc and 80Ëšc causing this area of my graph not to follow the pattern of my initial prediction. I have also noticed that the highest rate is at a slightly higher temperature than I expected‚ though this may be due to the large difference in temperature between each sample. As the temperature was increases the molecules were given more energy and move around in the solution quicker
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Lab Report DIFFUSION AND THE EFFECT OF AMYLASE. Aim: We will interpret and explain the activity of an enzyme added to a solution. We will analyze reactions in different pH ranges. The starch solution will appear blue when mixed with iodine. But after mixing the enzyme amylase to the starch‚ the starches are broken down into simple sugars. Now‚ when the iodine is added‚ no color change exist. Instruments and materials used: 1.) Dialysis bags x2 2.)
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