Testing Catalase Activity Prepared by Anel Behric For professor Goodrow General Biology Lab Mohawk Valley Community College 10/14/2014 6:00 PM Introduction: This experiment was conducted to examine the breakdown of substrate hydrogen peroxide (H2O2) by catalase‚ which is a specific enzyme that breaks down substrates of (H2O2). Molecules are in our bodies and nature. They move around constantly which causes them to be part of random collisions‚
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Investigating the effect of temperature on plant cell membranes The purpose of this activity is: • to practise experimental and investigative skills • to investigate the effect of temperature on cell membrane structures Procedure SAFETY: Always carry scalpels clasped to a tile and with the tip pointing away from you. Beetroot cells contain pigments called betalains that give the tissue its dark purple-red colour. The pigment is contained in the cell vacuole. Investigation
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until the enzyme reaches its optimum point of saturation‚ after which any increase in the substrate concentration will no longer affect the rate of reaction. The independent variable in this investigation is the varying concentrations of the substrate (Hydrogen Peroxide: 1%‚ 3%‚ 5% and 6%)The dependent variable was the rate of enzyme catalase activity‚ which was measured by the volume of froth produced after one minute.The concentration and volume of liver extract (source of catalase) used was kept
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Effects of Changes in Physical Properties on Enzyme Activity By Alex Hoffmann First Individual Lab Report Wednesday 7:30-10:15pm 10/24/12 Meghan Duell Abstract The goal of this lab was to determine the effects of certain physical properties on enzyme activity. Enzyme activity was measured by the height of the bubbles that appeared after the enzyme was added which are proportional to the rate of the reaction when time is constant. The fact that enzyme activity is affected by physical
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Usman Omid Biology IB 1A The Effect of Temperature on Beetroot Membranes _Aim_ This practical will test the effect of temperature on the integrity of the membranes‚ _Introduction_ The cells of beetroot contain a pigment called betalain in their vacuoles. It is kept inside the cells by the membranes. If these membranes are damaged‚ then the betalain leaks out. The amount that leaks out can be assessed‚ as the leaked out pigment will color the water surrounding the cells. This information can
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aim of this experiment was to determine the effect of caffeine on resting body temperature. This has to do with homeostasis‚ which is the maintenance of equilibrium. In other words‚ it is a stable body state. Homeostasis refers to the process of keeping the internal body environment in a steady state‚ when the external environment is changed (Homeostasis 2). Humans are warm-blooded creatures that generate body heat internally and maintain body temperatures at a fairly constant level – about 98.6oF
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EFFECT OF TEMPERATURE ON THE RATE OF CATALASE REACTION IN HYDROGEN PEROXIDE INTRODUCTION Enzymes function similarly to a lock a key mechanism where only one specific key will work (U and Fischer‚ 1994). An enzyme is a macromolecule that acts to increase the rate of either a substrate breaking down into two or more products‚ or where two substrates join up to form one product (Dundon‚ 2018). These enzymes perform this by lowering the activation energy required for the reaction to occur under normal
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4U1) Nicole Mikulis Unit: Biochemistry Sept 14 2012 Lab: Effect of temperature and pH on catalase activity BACKGROUND Catalase is an enzyme that detoxifies chemicals that might harm the cell such as hydrogen peroxide (H2O2). The enzyme breaks H2O2 into water and oxygen. The production of the oxygen gas bubbles serves as evidence that the catalase enzyme is working. As catalase is breaking the bonds between H2O2‚ it is releasing energy in the form of heat which
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In this experiment we are trying to determine the effect temperature has on catalase activity. We started out with five test tubes and then labeled them as we went about‚ after we added a catalase extract to each tube. Once added to each test tube they all sat in their respected temperature for ten minutes‚ so they could have a full effect. Once the time was up each was then collected and then we began to shake them from side to side for the foaming to start. When the foam settled‚ we discovered
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trend O2 production and reaction velocity increased with increasing catalase concentration‚ however‚ the 33% percent catalase concentration showed a drop of 0.175 mL O2/s compared to the 25% catalase concentration (figure 1.2). The velocity of 25% catalase was 0.275 mL/s‚ 33% was 0.1 mL/s‚ 50% was 0.435 mL/s‚ and 75% catalase was 0.575 mL/s (figure 1.1). The 50% catalase concentration produced the most O2 overall however the 75% catalase concentration had the fastest initial reaction velocity. Experiment
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