Investigation into how pH levels affect Catalase activity in potato samples – (enzymes) Introduction: The aim of this investigation is to understand and monitor the effects pH levels have on catalase activity within controlled potato samples. Catalase is a type of enzyme that is found widespread among organisms that grow in the presence of oxygen (almost all living things) its specific use is to speed up the decomposition of Hydrogen peroxide (H2O2) within cells. Hydrogen peroxide is a bi-product
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when different concentrations of catalase are added with hydrogen peroxide. Aim: To see if changing the concentration of catalase (found in celery) with hydrogen peroxide affects the amount of oxygen given of. Background Information: (Hydrogen peroxide - H2O2 1/2O2+H2O) Enzymes: Hundreds of chemical reactions happen simultaneously inside living cells and it’s the job of enzymes to control and regulate the various metabolic activities. Enzymes are biochemical catalysts‚ which
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Arnesh Kaewloyma 9/30/14 Enzyme Catalase under optimum conditions‚ speeds up the decomposition of hydrogen peroxide The students have studied that Hydrogen peroxide is a jeopardy to metabolism if it’s not obliterated. Enzymes are proteins produced by living cells of that act as catalysts which affects the rate of biochemical reaction by speeds up the breakdown of hydrogen peroxide into water and oxygen gas. The students have formed a hypothesis toward this experiment. If the speed of
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Hypothesis The optimum temperatures of Alcalase and Savinase will be different. Above and below their optimum temperatures activity will decrease. Biological explanation This investigation is designed to look at the effect of temperature on the activity of the proteases Alcalase and Savinase. By the end of it I hope to know the optimum temperature of both proteases. The substrate I am going to use during the experiments is the protein gelatin‚ which is a translucent‚ colourless‚ brittle solid
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of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but it would happen a lot more slowly because more energy
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study was to test the rate of reactivity of the enzyme catalase on hydrogen peroxide while subject to different concentrations of an inhibitor. The hypothesis was that hydrogen peroxide will be broken down by catalase into hydrogen and oxygen‚ where a higher concentration of inhibitor will yield less oxygen‚ resultant of a lower rate of reaction. Crushed potato samples of equal weight were placed in hydrogen peroxide solutions of various temperatures. The results showed that less gas was produced
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experiment to find the optimum temperature and pH for starch on amylase. The experiment was carried out in one day. The researcher and a partner did the experiment based on a lab manuel from class. Data was collected from the experiment and to be displayed on graphs. Then the optimum pH and temperatures were to be calculated based on the findings. The hypothesis was disproved due to the optimum pH of 5 but the other findings supported the hypothesis of the optimal temperature being 45 degrees Celsius.
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affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes; The enzyme catalase has an optimum operation condition
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Abstract In this lab‚ we tested the effects of temperature on fungal amylase and bacterial amylase (Aspergillus oryzae and Bascillus Licheniformis). We used 4 different temperatures in Celsius 0‚ 23‚ 58‚ and 89 for both fungal and amylase. For 10 minutes‚ every 2 minutes we would use 3 drops of each amylase and mix it with iodine to observe the presence of starch at each temperature. We conducted this experiment for both bacterial and fungal amylase. Results were reached based on the color of the
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This therefore supports my theory that at pH 7 the rate of reaction will be highest. This relate to other resources‚ such as books and the internet‚ which show that the optimum pH value for catalase is pH7‚ therefore there is no difference between the optimum pH and the pH used in the experiment‚ hence the enzyme structure is not affected by the pH and is most active. However the graph inadequately supports the second part of the prediction‚ that the rate will be lowest‚ the more acidic or alkaline
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