Investigation of the effect of metal ions on enzyme activity Objective To study the effect of two heavy metal ions‚ lead (II) ions and silver ions on the enzyme activity of invertase. Introduction Hypothesis: The rate of enzyme reaction of invertase decreases and the reaction eventually stops as the heavy metal ions inhibit the enzyme reactions. Biological principle: Heavy metal ions are non-competitive inhibitors. They do not compete with the substrates for the active sites of enzymes. They attach
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huge amounts of energy‚ and their molecules are spread out as much as possible. With very little pressure‚ when compared to liquids and solids‚ those molecules can be compressed. It happens all of the time. Combinations of pressure and decreasing temperature force gases into tubes that we use every day. You might see compressed air in a spray bottle or feel the carbon dioxide rush out of a can of soda. Those are both examples of gas forced into a smaller space than it would want‚ and the gas escapes
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Lab 4 Quiz 1. Enzymes and what is their function? a. Enzyme: biological catalysts that regulate chemical reactions that occur in cells. Responsible for things such as converting food to energy‚ replace old damaged tissues‚ disposal of cellular waste products. Are responsible for lowering the activation energy. 2. Most enzymes are proteins with three-dimensional shapes determined by their amino acid sequence. 3. Substrate – is a reactant molecule that binds to the highly specific active site
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Ching Man 5C(21) Effects of temperature on the activity of lipase Aim: To investigate the effects of temperature on the activity of lipase enzyme on milk which contain fats or lipids. Introduction: Enzyme is a kind of biological catalyst that made up of protein. It can speed up the metabolic reactions on various kinds of substances. Like lipase can break down lipid into glycerol or fatty acids in milk. Since enzyme is made up of protein which easily affected by varies temperature. This experiment
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Enzymes are organic catalysts; where a substance speeds up the rate of chemical reactions without changing being changed by the reactions. In lab they tested this by seeing how H2O2 and the catalysts from the banana and liver react to make H2O+O2. Depending on different conditions; like decomposition of H2O2 (surface area)‚ temperature on function‚ reusing the catalase‚ reaction rate of iced liver returned to room temperature‚ and effects of pH on enzyme activity‚ to see how much O2 was released
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The piece of metal is longer than the piece of wood b) Solution 1 is much darker than solution 2. c) The liquid in beaker A is blue. d) The temperature of the liquid is 60°C. e) At least two of these (a-d) are quantitative observations. ANS: d) The temperature of the liquid is 60°C. Page: 1.2 5. A quantitative observation a) contains a number and a unit. b) does not contain a number. c)
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BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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introduction to enzymes Enzymes are the foundation of energy and the life force in all living things. They are responsible for building‚ detoxifying‚ and healing the body. They are also the force that allows your body to digest and absorb food. Enzymes also regulate tens of thousands of other biochemical functions that take place in the body every day. Without enzymes‚ seeds would not sprout‚ fruit would not ripen‚ leaves would not change color‚ and life would not exist. Therefore‚ the study of enzymes has
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Enzyme Immobilization Methods Covalent Binding: Covalent binding is a conventional method for immobilization; it can be achieved by direct attachment with the enzyme and the material through the covalent linkage [37]. The covalent linkage is strong and stable and the support material of enzymes includes polyacrylamide‚ porous glass‚ agarose and porous silica [38]. Covalent method of immobilization is mainly used when a reaction process does not require enzyme in the product‚ this is the criteria
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Kenneth Hampton | | |Restriction Enzymes: | |A study in Reactions and Mapping | | | |November 7‚ 2008 | ABSTRACT This experiment will study the
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