pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because we assume the more substrate an enzyme gets
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aim of this investigation is to determine how the concentration of hydrogen peroxide effects the intensity of the reaction. Variables: Independent- The concentration of hydrogen peroxide. Dependant - The intensity of the reaction. Constants – The temperature‚ the amount of liver‚ pH level‚ test time‚ Materials: Solutions of hydrogen peroxide varying in concentration 1‚2‚4‚6%‚ fresh liver(containing catalase)‚ sharp knife‚ forceps‚ cutting tile‚ 2x 10mL measuring cylinder‚ 1x 100ml measuring
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ENZYME STRUCTURE AND FUNCTIONS: Enzymes are biological catalysts. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures Definition of enzyme: A protein with catalytic properties due to its power of specific activation is defined as an enzyme. STRUCTURE Enzymes are proteins their function depends on its complexity. The reaction takes place in a small part of the enzyme
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the climate of a region is an important factor in determining which organisms can survive there. Even within a biome‚ precise conditions of temperature and precipitation can vary over small distances. Ecologists recognize at least ten different biomes. The world’s major biomes include tropical rain forest‚ tropical dry forest‚ tropical savanna‚ desert‚ temperature grassland‚ temperate woodland and shrub land‚ temperate forest‚ northwestern coniferous forest‚ boreal forest‚ and tundra. A unique set of
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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Enzymes- reduce the amount of energy required for the reactions they catalyse [accelerate]. -thus‚ increasing the rate of reactions that occur in living organisms. WITHOUT enzymes : metabolism would be so slow at body temp. that insufficient energy would be available to sustain life. -Many enzymes are “Intracellular”- used within the cell that produced them e.g. enzymes in respiration & photosynthesis -Others are “extracellular”-they act outside the
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Effecting the Reaction Rate in Enzymes in Plants and Animals Problem- How do variables temperature and surface area affect the rate of reaction occurring in the enzyme? Information- Enzymes are proteins that act as biological catalysts. They speed up chemical reactions that take place in cells. Enzymes can be found in both liver and potato. They provide a site where reactants can be brought together to react. These reactants are called substrates that fin into the enzyme. The changing of heat‚
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Lab Report Enzymes (must be typed) Name: Amanda Gallegos Date: 2/15/15 Section: BIOL 101GL What is the Hypothesis of this experiment? (3 points) -The highest temperature will affect the reaction rate of enzymes. What is the dependent variable? (1 point) -Change of rate/product/color/absorbency. What is the independent variable? (1 pt) -The temperature. Explain in detail the procedure that you followed (including amount of substrate‚ enzyme etc‚ and the whole procedure including incubation times)
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The Effect of Substrate Concentration on Initial Reaction Rate The purpose of this study is to identify the relationship between substrate concentration and initial reaction rate provided that substrate concentration is much greater than enzyme concentration. Enzymes are essential to life as they are required for many vital metabolic reactions to occur. To adequately explain the properties of enzymes‚ it is assumed that an enzyme-controlled reaction takes place through an enzyme-substrate
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