Effects of pH on the Function of Enzymes Rena Mototani Glen Rock High School Advanced Biology 2014-2015 Effects of pH on the Function of Catalase Rena Mototani Problem: How does the pH of a cell affect the function of the enzyme catalase? Introduction In this lab‚ we experimented the effects of pH on the function of the enzyme catalase. Catalase is an enzyme that brings about the reaction by which hydrogen peroxide is decomposed to water and oxygen (Encyclopedia Britannica). The
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3.8.3. Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature
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LABORATORY REPORT Activity: Enzyme Activity Name: Daniel Franco Instructor: Professor Jennifer Frere Date: 03.08.2015 Predictions Sucrase will have the greatest activity at pH 6 Sucrase will have the greatest activity at 60 °C (140 °F) Sucrase activity decreases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of substrate
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The Effect of catalase reaction on temperature Table of Contents: Bibliography ………………………………………………………………………………………………….. Page 1 Introduction……………………………………………………………………………………………………Page 2-3 Methods/ Procedures……………………………………………………………………………………..Page 4-5 Results ………………………………………………………………………………………………………….. Page 6 Analysis of Data ……………………………………………………………………………………………. Page 6 Graph …………………………………………………………………………………………………………... Page 7 Conclusion……………………………………………………………………………………………………
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Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins which cut dsDNA at specific regions depending on the enzyme used‚ determined by the nucleotide sequence of the DNA‚ i.e. each enzyme recognises specific nucleic
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Awards/Honors‚ Health Care Exposure‚ Community Service‚ Research‚ Leadership‚ Extracurricular Activity‚ Employment‚ and Other. In the "Description of Role" section of each activity‚ please provide additional details regarding how it correlates with any other "Activity Type." The admissions committee takes into account the fact that some activities may fit multiple categories. Activity Type: Health Care Exposure Activity Name: Pharmacy Technician Organization Name: CVS pharmacy Contact/Supervisor Name: Dina
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Abstract: After reviewing the basics of enzymes and catalysis‚ we take a dive into the wonderful world of catalase. Beginning with establishing a base line of just how much hydrogen peroxide there is in 5.0mL of the reacted solution; to figuring out exactly how much actually reacted after 300 seconds of catalyzed reaction. Follow the experiment from the beginning steps right to the end as you see where the students went wrong‚ interpretation of the results‚ and great answers to work sheet
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Investigating the Effect of Concentration on the Rate of Enzyme Catalysed Reaction To investigate the effect of concentration on the rate of enzyme catalysed reaction I must know all the factors that affect it in order to investigate in this. Here are all the factors: Temperature- Enzyme like it warm but not too hot. Enzymes are biological catalysts‚ which speed up chemical reactions. They work most efficiently at the optimum point (37°C). If they are below 37°C they will work
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Lab #5: Dissolved Oxygen Lab Be sure to read and understand the below instructions BEFORE the lab! Experiment 1: Investigating the Effect of Temperature and Salinity on the Concentration of Dissolved Oxygen in Water Introduction: In an aquatic environment‚ oxygen must be in a solution in a free state (O2) before it is available for use by organisms (bio-available). Its concentration and distribution in the aquatic environment are directly dependent on chemical and physical factors and are
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Enzymes are catalytic proteins which speed up the rate of reactions. Every enzyme has a specific function – meaning‚ they can only bind to certain substrates. Because these enzymes are proteins‚ they can be denatured. Enzymes can be denatured by many factors‚ such as pH and temperature. This lab was divided into three parts which examined the effects of pH‚ enzyme concentration and temperature on the rate of which enzymes catalyze. The pH is an index of hydrogen ions. In acidic conditions‚ where
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