Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins which cut dsDNA at specific regions depending on the enzyme used‚ determined by the nucleotide sequence of the DNA‚ i.e. each enzyme recognises specific nucleic
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BIOLOGY HSC NOTES MAINTAINING A BALANCE • Identify the role of enzymes in metabolism‚ describe their chemical composition and use a simple model to describe their specificity on substrates. Enzymes are protein molecules that allow the body to engage in chemical reactions‚ such as metabolism. There activities can be catalytic (being able to control the rate of either increasing/decreasing chemical reaction) Enzymes have a specific shape‚ and this shape must be intact‚ otherwise the effectiveness
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Enzyme Lab Report Introduction The objective of this experiment was to determine if changes in pH or temperature affected the activity of enzymes‚ specifically the enzyme sucrase. Enzymes are protein molecules that act as biological catalysts to increase the speed of the reaction or to lower the activation energy of that reaction. However‚ the activity of an enzyme can be affected by physical factors such as pH and temperature because these factors alter the structure of the enzyme (Freeman‚ 2011)
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Introduction: Enzymes are biological catalysts that permit cells to carry out the many functions that are required in a living system. Every enzyme has a specific substrate and a specific function. Enzymes alter substrates one of three ways: by adding something to the substrate‚ removing something from the substrate‚ or by changing its conformation‚ otherwise known as its shape. The structure of an enzyme and its ability to function exists because it binds to an active site‚ thus increasing the
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_____ 3. Questions 15 points/ _____ Lactase enzyme lab OBJECTIVES: 1. Understand how biological molecules are essential to the survival of living organisms * * 2. Compare the structures and functions of the major biological molecules(carbohydrates‚ proteins‚ lipids‚ and nucleic acids) as related to the survival of organisms. * 3. Investigate and describe the structure and function of enzymes and explain their importance in biological systems
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Abstract: After reviewing the basics of enzymes and catalysis‚ we take a dive into the wonderful world of catalase. Beginning with establishing a base line of just how much hydrogen peroxide there is in 5.0mL of the reacted solution; to figuring out exactly how much actually reacted after 300 seconds of catalyzed reaction. Follow the experiment from the beginning steps right to the end as you see where the students went wrong‚ interpretation of the results‚ and great answers to work sheet
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All living organism depend on catalytic proteins called enzymes‚ these compound are responsible for metabolism and biochemical regulation of energy‚ there are millions of enzymes and hundreds of enzymes have been identified in the human body as well. All living system possess enzymes as vital components of the body‚ enzymes are found in all organisms. Everyone has a natural pH balance in the body. Enzymes are complex proteins which speed up chemical reactions if this did not happen it would occur
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Patrick McCrystal Enzymes: Natural Catalysts Enzymes are catalytic proteins‚ meaning they speed up chemical reactions without beingused up or altered permanently in the process. Although various enzymes use different methods‚all accomplish catalysis by lowering the activation energy for the reaction‚ thus allowing it tooccur more easily. Enzymes have very specific shapes (conformations). Part of the conformationis the active site of the enzyme‚ where the actual catalysis occurs. The specific molecule
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Enzyme Catalysis Introduction: Enzymes are produced by living organisms as proteins. These enzymes perform as catalysts to bring about a chemical reaction. In fact‚ most reactions are catalyzed by enzymes during reactions in the cell or in the human body. A catalyst that enzymes pose ad are by definition substances that are capable of initiating or speeding up a chemical reaction. Catalyst are not a necessity during a chemical reaction‚ they are just used to speed up a chemical reaction. This event
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Investigating the Effect of Enzyme Concentration on Pectinase in the Production of Fruit Juice In this practical‚ I shall demonstrate the relationship between the concentration of pectinase and the amount of fruit juice produced accordingly in proportion. Apparatus: Apples Pectinase solution 10 x filter paper Knife or kitchen mincer Glass rod Water bath X 1 250 ml beakers X 2 Wash bottle of distilled water 100 cm^3 measuring cylinders In the case
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