Trevor Smith Biol 4400 Dr. Achberger May 8‚ 2013 a. From the amino acid sequence the gene is the LacI repressor for Escherichia coli. See appendix 1 for the search result. b. The complete nucleic acid sequence of the protein with the open reading frame (ORF) indicated by red and green colorization as well as the forward and reverse primers underlined (a screenshot was taken so the sequence could be clearly displayed): c. The ORF and flanking nucleotide sequences are displayed in part “b
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OBJECTIVE: The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different
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The purpose of this lab was to observe the rate of osmosis and diffusion‚ as well as the effect of molecular size of the particles on this rate. Part I of the lab was a demonstration of osmosis and diffusion‚ that dealt with raisins in different liquid environments‚ each with a different concentration of sugar. Part IV of the lab was using the same idea as the demonstration‚ by putting objects in different concentrations of a substance; in this case elodea leaves in salt water. In both cases‚ the
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Hands On Approach to Diffusion and Osmosis Abstract A. Plant and Animal Cell Diffusion and Osmosis In this experiment we observed carmine powder to better understand the concept of Brownian motion and the role of molecular movement in diffusion. The particles continuously moved in a completely random fashion‚ and the smaller particles moved with more speed than the larger particles. B. Potato Core Experiment In this experiment we estimated the osmolarity of potato tuber tissue
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therefore‚ it would take longer for a person to decide to call for help and the longer span of time taken can lead worse injuries and even the death of those involved in the accident. An experimental research study that would help test whether diffusion of responsibility is present
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Characteristics of Gases Section 10-1 The Kinetic-Molecular Theory of Matter Prerequisites The kinetic-molecular theory is based on the idea that particles of matter are always in motion. The theory can be used to explain the properties of solids‚ liquids‚ and gases in terms of the energy of particles and the forces that act between them. ideal gas: an imaginary gas that perfectly fits all the assumption of the kinetic-molecular theory. Kinetic-Molecular Theory of Gases (based on the following
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THE EFFECT OF THE WATER CONCENTRATION GRADIENT ON THE RATE OF OSMOSIS Investigation Folio: Summative Stage 1 Biology: SEMESTER 1 2014 MARCIA SHAW - 151003X INTRODUCTION Diffusion: is when something spreads more widely from a higher concentration gradient to a lower concentration gradient. Diffusion is the process from which molecules‚ atoms or ions mingle together and move from an area of high concentration to an area of low concentration as a result to their kinetic energy. for example
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mechanisms that regulate cellular senescence‚ organismal ageing‚ and species-specific lifespan depend on a synergy of pathways that are multifactorial and extremely complex‚ though not yet completely understood. Recently‚ the development of new molecular techniques has elucidated‚ at least in part‚ the primary pathways involved in ageing. In parallel with the search to uncover the factors that control ageing is the endeavor to discover methods of extending lifespan‚ in hopes of living both youthfully
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The Effect of substrate on the Rate of Respiration on Yeast1 Justine Maturan Group 4 Sec. Y – 5L November 18‚ 2014 ________________________________________________________________ 1A scientific paper submitted in partial fulfilment of the requirements in General Biology I Laboratory under Prof. Susan Sedano‚ 1st semester 2014-2015 ABSTRACT In order to determine the effect of the substrate on the rate of respiration of yeast‚ Durham test tube method was used in the first experiment
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Investigate the effect of on exercise on the heart rate. Aim: To find out if there is any relevant link between the amounts of exercise taken to increase a person heart rate. Equipment needed: Stop watch Screen volunteer P.E kit Calculator Record sheet Type of exercise: skip for few minutes ‚ then break in between and again repeat it once or twice. Method: First of all‚ we needed to take the resting heart rate of the person who is about to skip. Then‚ with the stopwatch one
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