Lab #6: pH Effects and Enzyme Concentration on Catecholase Activity Abstract: This experiment examined the effect on how the changes in pH balance of potato juice affected the enzyme activity‚ structural stability and solubility. Introduction: Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in‚ but not changed by or consumed in‚ a chemical reaction. The amount of energy required for a reaction
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are studying the effect of pH on catalase activity. Catalase by scavenging hydrogen peroxide to water and oxygen is an important enzyme of cell defense mechanisms against oxidative stress in all living organisms (Dat et al.‚ 2003). Cells use catalase because it is the most efficient enzyme as an antioxidative enzyme which lowers hydrogen peroxide or superoxide to accumulate to toxic levels in plant growth (Bowler et al. 1992). The formula that involves catalase as the enzyme is . pH is a measure of
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EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz‚ Michael Ray Dela Cruz‚ Karen Angelica Domalaon‚ Abegail Dulay‚ Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor
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Objective: To investigate how pH affects the rate of protein digestion Hypothesis: I predict that since pepsin breaks down the material the quickest at a pH of 2 in the stomach‚ test tubes #3 will reduce the greatest because it has pepsin to further break down the egg. Whereas the other test tubes will have a smaller reduction. I think test tube #2 will have the second largest reduction rate and then #6 because they all will have a greater rate at breaking down the material‚ Then‚ I predict the
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What effect does crowding have on bean plants? My prediction was that crowding would have no effect on them. I predicted it this way because plants are made to grow in tough conditions and I dident think crowding would have any effect. Materials needed to experiment. 2 Planting pots Planting soil 4 Bean seeds Water Ruler Support stick Notebook Procedure: Take your planting pot fill with planting soil a little above half way. Take your seeds but before you plant
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Effect of pH on Enzyme Activity Athenaa Jeyachandran Mr.Menes Course Code January 21st‚ 2013 The Effect on Enzyme Activity Due to Temperatures Purpose: The purpose of this lab experiment is to examine how different temperatures affect enzyme activity using hydrogen peroxide. Hypothesis: My hypothesis for this experiment is that temperatures near body temperature is when enzyme activity will be at its highest. I believe this will occur because in our body‚ enzymes
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How does pH affect the activity of the Amylase? Abstract: In my experiment I aimed to observe how ranging pH levels will affect the rate in which amylase will break down the starch molecules. I will be measuring the time it takes for the dark liquid to disappear and leave a yellow brown liquid to be shown‚ which would show that there is no starch present in the solution because it would have broken into maltose by adding amylase. Results did not fully demonstrate what we expected in our hypothesis
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pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
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Effect of Sugar on Bean Plant Growth Abstract The objective: My project was to determine if bean plants grew stronger and healthier by the addition of the right amount of sugar to their watering. I believe that plants that receive 50 grams of sugar per liter of water would help bean plants grow to be stronger‚ healthier and larger because they would get energy from the sugar. Methods/Materials 36 bean plants were grown in potting soil. The same amount of soil was used in each pot and it had no added
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Title: Amount of Oxygen‚ Mung Beans (Vigna Radiata) Consume during Cellular Respiration Introduction: Cellular respiration is a catabolic reaction that refers to the process of converting chemical energy of organic molecules into a simplify form so it can be used immediately by organism. Glucose may be oxidized completely if sufficient oxygen is available‚ by the following equation: C6H12O6 + 36 ADP + 36Pi + 6O2(g) 6 H2O + 6 CO2(g) + 38 ATP + heat All organisms‚ including plants and animals
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