the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started to form on the culture distinguishing Staphylococcus spp. form on the culture distinguishing Staphylococcus spp. from Streptococcus spp. Finally‚ a Mannitol Salt Agar
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Melissa Carrithers AP Chemistry 12/17/14 Rate Law Determination Pre-Lab Purpose: The purpose of this lab is to observe the reaction between sodium hydroxide and crystal violet so that the order of the reaction could be determined along with the rate constant‚ k‚ and the half-life for this reaction. Hypothesis: If the reaction between crystal violet and sodium hydroxide reacts appropriately‚ then the order will be first order. Procedure: Mix 10 mL of sodium hydroxide and crystal violet solution
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The Effects that 1%‚ 4%‚ and 16% Sodium Chloride (NaCl) Concentration Had on the Rate of Reaction of Catecholase Enzymes in a Potato (Solanum tuberosum). Abstract Enzymes are a key component of a cell. They make chemical reactions happen faster because they lower the activation energy to make the chemical reaction occur. Most of the time‚ it is best if enzymes produce as efficiently as possible‚ but in some cases it is better if they do not‚ when dealing with potatoes (Solanum tuberosum).
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Effect of temperature and pH on catalase activity BACKGROUND Catalase is an enzyme that detoxifies chemicals that might harm the cell such as hydrogen peroxide (H2O2). The enzyme breaks H2O2 into water and oxygen. The production of the oxygen gas bubbles serves as evidence that the catalase enzyme is working. As catalase is breaking the bonds between H2O2‚ it is releasing energy in the form of heat which is reason for the warmth of the bubbles during the reaction. Starch acts to trap these
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Factors that affect climate Latitude: Ocean Currents: Wind and air masses: Elevation: Relief: Near water: Global Factors that Affect Climate As the suns UV rays hit the earth they hit is directly at the equator causing more heat. Because these rays are direct and they hit direct at one spot. For part a‚ the earth start to slope‚ in which the sun’s rays have to hit a larger areas‚ and have to travel further distances. As the latitude increases‚ the average annual temperatures decreases. In North
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of pH on catalase activity. Catalase by scavenging hydrogen peroxide to water and oxygen is an important enzyme of cell defense mechanisms against oxidative stress in all living organisms (Dat et al.‚ 2003). Cells use catalase because it is the most efficient enzyme as an antioxidative enzyme which lowers hydrogen peroxide or superoxide to accumulate to toxic levels in plant growth (Bowler et al. 1992). The formula that involves catalase as the enzyme is . pH is a measure of the Hydrogen ion concentration
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Experiments on the Enzyme Catalase Aim: The aim of this practical is to use three different techniques to investigate the effect of different concentrations of the enzyme catalase on the rate of breakdown of hydrogen peroxide. Background information Catalase is an enzyme which is found in all living organisms. This enzyme catalases the decomposition of hydrogen peroxide into water and oxygen. Cells continually produce a poisonous by-product of metabolising‚ called hydrogen peroxide. This is very damaging
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Investigating The Rate Of Reaction Between Marble Chips And Hydrochloric Acid Planning I will use marble chips and different concentrations of hydrochloric acid and water to see how it affects the rate of reaction. I will use concentrations of 50cm3 using different volumes of water and acid. Introduction I am investigating how the rate of reaction between marble chips and hydrochloric acid is altered when the concentration is changed. This is the word equation for my investigation
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THE EFFECT OF TEMPERATURE ON CATALASE RESEARCH QUESTION: What is the effect of various temperatures‚ 0°C‚ room temperature‚ 37°C‚ 50°C‚ 60°C‚ on the number of oxygen gas bubbles liberated‚ in a decomposition reaction between the enzyme Catalase‚ obtained from crushed mung beans‚ and 2% of the substrate Hydrogen Peroxide? INTRODUCTION: Enzymes are biological catalysts that increase the rate of chemical reactions without they themselves being involved in the reaction itself. Enzymes are proteins
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of 1% hydrogen peroxide solution was made. In order to get this‚ the stock solution‚ which was 3%‚ was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker‚ a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay was performed. Using forceps a small paper disk was dipped into the second beaker containing the potato catalase for
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