Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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[Hide] [Help us with translations!] | DNA vaccine What is antisense technology? Antisense refers to opposing the normal order (“sense”) of the code in DNA. The DNA (deoxyribonucleic acid) in genes directs cells to assemble the proteins which comprise living creatures. The order of bases in DNA corresponds to the ordering of amino acids to form the proteins. To produce protein‚ the DNA of the genes in cells is first transcribed into a very similar molecule called RNA. RNA can move out of the cell’s
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The upper most strand is the (coding strand) DNA base sequence (triplet) of the gene codes for synthesis of a particular polypeptide chain. The second strand is the mRNA base sequence (template strand used for copying) codon of the transcribed mRNA. The process for going from the upper stand to the second strand is called Transcription and involves an enzyme called polymerase. The polymerase attaches to the promoter region (start codon) and reads the nucleotide base sequence until it gets to a termination
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MOLECULAR STRUCTURE OF DNA AND RNA •Adapted from Chapter 9 in Genetics: Analysis and Principles (Robert J. Brooker) Copyright ©The McGraw-Hill Companies‚ Inc. Permission required for reproduction or display IDENTIFICATION OF DNA AS THE GENETIC MATERIAL • To fulfill its role‚ the genetic material must meet several criteria – 1. Information: It must contain the information necessary to make an entire organism – 2. Transmission: It must be passed from parent to offspring – 3. Replication:
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The Advantages of DNA Replication DNA stands for Deoxyribonucleic acid‚ and it is found in the nucleus of every cell in the human body. DNA is the master plan - it contains all the genetic information needed for a living thing to develop and function. Each and every single organism has a unique and different DNA encoding. DNA’s purpose is to store all of our genetic information similar to the way a builders blue print sets out the design of a house. Without DNA there would be no such thing as
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Legal Base US-Cuba Business History The well-known Unites States (US) embargo against Cuba is generally related to the 1960 when the Cuban government under Fidel’s Castro wake‚ seized private land‚ privates companies and taxed the US products in excess. But this harsh relation dates back from 1898 when‚ after the Spanish-American war‚ Spain cede the Puerto Rico‚ Guam and Cuba territories to the US. The US granted Cuba its independence with the condition that it could intervene in the country
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The identification of acids and bases using a natural indicator Purpose: The purpose of this lab practice is to identify the pH of certain solutions and if they are acids or bases. Introduction: In chemistry‚ pH is a measure of the activity of the (solvated) hydrogen ion. p[H]‚ which measures the hydrogen ion concentration‚ is closely related to‚ and is often written as‚ pH. Depending on the pH solutions will be acids or bases. An acid is a chemical compound that dissociates in solution‚ releasing
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Exercise 10: Acid/Base Balance Worksheet Assignment Due: Week 1 Student instructions: Follow the step-by-step instructions for this exercise found in your text and record your answers in the spaces below. Submit this completed document by the assignment due date found in the Syllabus. Please make sure that your answers are typed in RED. Please type your Bobby Rivera and 4023166: Respiratory Acidosis and Alkalosis Activity 1: Normal Breathing 1. At 20 seconds‚ pH = 7.40
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based upon one thing that unites us all‚ DNA. DNA‚ standing for Deoxyribonucleic Acid‚ is commonly compared to a blueprint. This blueprint orders a highly specific set of corresponding bases‚ which in turn codes for an amino acid. However‚ this specificity comes at a very high risk; a single error in a base could code for a completely different amino acid‚ causing a mutation that could devastate the organism. Recently‚ scientists have discovered a new method to edit and repair DNA that could hopefully
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BioLab3 DNA Structure and Function Lab Report Student Name: I. DNA Structure Define the following terms: Purines A colorless crystalline compound with basic properties‚ forming uric acid on oxidation. Pyrimidines A colorless crystalline compound with basic properties; a substituted derivative of this‚ especially the bases thymine and cytosine present in DNA. Nucleotides A compound consisting of a nucleoside linked to
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