Determining the Concentration of an Unknown Protein Solution with the use of the Bradford Method Paul Alcala‚ Andrea Basa‚ Melissa Caagbay‚ Frances Cayomba Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state
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Bonnie Smith does achieve most of the stuff she set out to do in her book such as illuminating the evolution of sexism in western historiography and the varying definitions of masculinity and femininity. To demonstrate her points‚ Bonnie Smith uses naïve tales of queens and famous ladies of the past to show how women were viewed as superficial and trivial in the past. She uses this to analyse women’s work and to show their amateur status in historical studies. Bonnie Smith used the language of sexuality
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Soldier‚ prisoner‚ slave and explorer… All these titles belong to one man: John Smith‚ a historical figure who played a huge role in the establishment of the Jamestown Colony. Remarkably‚ there were two instances that Smith was rescued from death at the last moment by a love struck princess‚ one of which involved the iconic Pocahontas who is also a big part of history. Smith tells the exchange like this‚ “…and thereon laid his head‚ and being ready with their clubs‚ to beat out his braines‚ Pocohontas
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In his notable novel‚ The Stone Face‚ author William Gardener Smith reveals the complications that arise when one’s ideals are questioned and put into focus. This complexity is demonstrated through the character of Simeon Brown as he tries to negotiate the condition of his racial ideal. However‚ the complex nature of his conviction poses a challenge that he struggles to overcome. As he makes every effort to understand the principle of race in his life‚ Simeon essentially grows to recognize the true
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General History of the Things of New Spain Bernardino de Sahagún And after the dying in Cholula‚[1] the Spaniards set off on their way to Mexico‚[2] coming gathered and bunched‚ raising dust.... Thereupon Moteucçoma[3] named and sent noblemen and a great many other agents of his...to go meet [Cortés]...at Quauhtechcac. They gave [the Spaniards] golden banners of precious feathers‚ and golden necklaces. And when they had given the things to them‚ they seemed to smile‚ to rejoice
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Bradford Protein Assay Practical Report 1. Present your data (including raw data and calculated concentrations) for the protein standards in the form of a clear table. Give one example of how you calculated protein concentration. Do not forget a descriptive title and units (4marks) Title either too long or not descriptive or absent Your results are in duplicate & shouldn’t be referred to as ‘set1 & set2’ or ‘original’ and ‘duplicate’ Many of you think units of absorbance are nm but A has arbitrary
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John Smith and Benjamin Franklin were both remarkable men known for their writings and works. There are many similarities between these two men‚ such as they were both authors. There are also many differences. John Smith “served as a foot soldier for the English in the Scottish Lowlands” (pg.16) and he was also an adventurer. Benjamin Franklin was a “-scientist‚inventor‚ educator‚ statesmen...and foremost a printer.” (pg.94). Here are some of the comparisons between Franklin and Smith. As I had
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Myrtles Plantation Haunting Imagine being jolted awake in the middle of the night only to see a ghostly figure staring at you by the foot of your bed. This is what many people witness while staying at the Myrtles Plantation Bed and Breakfast located in beautiful St. Francisville‚ Louisiana (Nickell 12). The Plantation house is split into two parts‚ a museum and sleeping quarters for the brave guests who dare to stay the night (Rule 238). The Myrtles Plantation is known as one of “America’s Most
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variety of different reasons‚ including‚ searching for economic prosperity‚ religious freedom‚ or to create a better life for themselves by escaping persecution. The colonies of Plymouth and Jamestown are an excellent example of the diverse cultures and reasons behind why colonies were formed. Although Jamestown and Plymouth were alike and suffered many of the same hardships‚ their way of life was completely different. Whether the colony suffered or prospered therein fell with how prepared the colonists
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arisen from the Myrtles Plantation. However‚ which are actually true? To understand some of the legends behind the plantation‚ you should understand the history behind it. The Myrtles Plantation is an antebellum plantation that was built around 1796 and 1797 by General David Bradford‚ also known as “Whisky Dave” in St. Francesville‚ Louisiana (Kermeen 43). Bradford lived there alone for a while. He then moved his wife‚ Elizabeth‚ and his five children to the Louisiana plantation from the northeast. Once
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