Dr. GEORGE ROMBO Cholera This is an infection of the small intestine caused by bacterium vibrio cholera. Transmission is mostly from the fecal contamination of food and water that is caused by poor sanitation Susceptibility About 100 million bacteria must typically be ingested to cause cholera in a normal healthy adult. Children are also more susceptible with 2- 4 year old having the highest rates of infection Individual susceptibility to cholera is also affected by their blood type with
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Microbiology Final exam Study reference 1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates
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Foundations in MicrobiologySeventh Edition 13.1 We Are Not Alone The human body exists in a state of dynamic equilibrium Many interactions between human body and microorganisms involve the development of biofilms Colonization of the body involves a constant “give and take” Contact‚ Colonization‚ Infection‚ Disease Microbes that engage in mutual or commensal associations – normal (resident) flora ‚ indigenous flora‚ microbiota Infection – a condition in which pathogenic microbes penetrate
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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What is Food Microbiology Food microbiology is the study of food micro-organisms; how we can identify and culture them‚ how they live‚ how some infect and cause disease and how we can make use of their activities. Microbes are single-cell organisms so tiny that millions can fit into the eye of a needle. They are the oldest form of life on earth. Microbe fossils date back more than 3.5 billion years to a time when the Earth was covered with oceans that regularly reached the boiling point‚ hundreds
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unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge knowledge and skill in performing laboratory techniques‚ ability to critically evaluate the information obtained from
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BTN 211 TOTORIAL 1 Chapter 1 1. The History and Scope of Microbiology Which of the following distinguish the field of microbiology from other fields of biology? a. the size of the organism studied b. the techniques used to study organisms regardless of their size c. both the size of the organism studied and the techniques employed in the study of organisms d. neither the size of the organism studied nor the techniques employed in the study of organisms regardless of their size Who of
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Biology 2020 Microbiology Study Guide for first exam The history and scope of microbiology 1) What are the organisms/entities of study in the field of microbiology? * Bacteria * Archaea * Eukarya * Viruses and Prions (Acellular entities) 2) Who was the first human to publish extensive descriptions of microorganisms? * Antony van Leeuwenhoek 3) How did Louis Pasteur dismantle the theory of spontaneous generation in bacteria? * Swan neck flask experiment: Flask
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Bibliography: Black‚ Jaquelyn G.‚ Microbiology: Principles and Explorations‚ 7th Edition‚ John Wiley & Sons‚ Inc‚ Hoboken‚ NJ‚ 2008 Davis‚ Charles P.; Marks‚ Jay W.‚ (2011) E. coli 0157:H7 (Escherichia coli 0157:H7 infection). Retrieved from: http://www.medicinenet.com/e_coli__0157h7/article.htm Morello‚ Josephine A.‚ Mizer‚ Helen Eckel‚ Granato‚ Paul A.‚ Laboratory Manual &Workbook in Microbiology: Applications to Patient Care‚ 9th Edition‚ McGraw-Hill Higher Education
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