Abstract Enzymes are proteins that lower the activation energy needed for chemical reactions. The two main environmental factors that can affect the enzyme’s activity are temperature and pH‚ and each enzyme works best at a particular temperature and pH. The purpose of this enzyme kinetic experiment was to observe the effect of temperature and pH on the reaction of barley alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature
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The main focus of this experiment was to find the relationship between temperature and the enzyme activity of amylase. This was achieved by attaining amylase enzyme‚ starch solution and potassium iodide (determines if enzymes hydrolyses the starch solution)‚ water bath and a hot plate. The temperatures used for this experiment were room temperature‚ 37oC‚ 60oC‚ 80oC‚ and 90oC. The hypothesis developed was that as the temperature increased‚ so will enzyme activity. Therefore‚ the ability of the
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was placed in carbohydrate solution at different temperature to identify unknown carbohydrate solution. The enzyme that involved in the reaction is salivary amylase. The action of amylase on starch is with the mixture of IKI (iodine and potassium iodide test).Starch solution will have a blue-black colour in the mixture but no changes in colour for maltose solution. When hydrochloric acid (HCl) is added to the starch solution‚ the color will
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the flask‚ while swirling it. During adding the mixture‚ the temperature was kept between 40 degrees C-50 degrees C. The process took 15-20min. Once the 20 minutes was up‚ the mixture was tested by dropping some of the mixture onto a piece of starch iodide paper. After the test‚ the reaction was transferred to a 250mL round bottomed flask and was set up for simple distillation. To help with distillation‚ boiling chips was added. The distillation was continued until 40mL was collected. Experiment 6
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III. Purpose: 1-To practice redox titration. 2-To determine the amount of vitamin C present in vitamin C tablet. IV.Equippments‚tools‚chemical reagent: -starch solution 0.2% -potassium iodate KIO3 -potassium iodide KI -sulfuric acid H2SO4 0.6 M -sodium carbonate Na2 CO3 -vitamin C tablet -sodium thiosulfate Na2S203 -analytical balance -10 ml and 25 ml pipettes equipped with pipette bulb - burette
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Title: Today’s Human Malady: the Nuke plant’s radiation Japan’s nuclear plant in Fukushima‚ northeast of Tokyo‚ had been the latest scare for the past few weeks. It seems that the nuke’s condition is worsening. First‚ it there was just a leak‚ then it turned into a hydrogen explosion and now it is a partial meltdown. People fear the dangers of radiation especially now that it is reported that there are considerably high radiation to Japan’s water and vegetables. It is important that the public
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QUALITY CONTROL LABORATORY Mam Dawn Assay of HCL Aqueous Alkalimetry Direct titration 0.1N NaOH Methyl Red TS HCL + NaOH NaCl + H2O Assay of Diluted H3PO4 Aqueous Alkalimetry Direct titration 0.1N NaOH Thymolphthalein TS H3PO4 + 2NaOH Na2HPO4 + 2H2O Assay of H3BO3 Aqueous Alkalimetry Direct titration 0.1N NaOH Phenolphthalein TS H3BO3 H+ + BO2- + H2O
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Under a month of dry and hot spell‚ Kuala Lumpur has reached the second highest temperature ever recorded (38⁰C) on Tuesday‚ 4th of March. Prolonged dry season with below average precipitation is the natural cause for drought. The state government has taken a right action by implementing water rationing on two days interval at affected areas. Although water rationing can help to conserve water in the dams‚ it could not solve drought problems forever. Severe drought brings greatest impact on agricultural
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Title: Quantitative analysis of vitamin C contained in ribena Aim: to determine the amount of Vitamin C in a sample of ribena Materials: * Vitamin C (ascorbic acid‚ C6H8O6) * 4% potassium iodide (KI) * 1M acetic acid (CH3COOH) * N-bromosuccinimide (NBS‚ C4H4BrNO2) Apparatus: * Conical flask * 100 ml volumetric flask * Weighing bottle * Analytical weight balance * Spatula * Funnel Results: Item: | Mass in grams | Mass of weighing bottle with ascorbic
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Class X Question 1: Chapter 1 – Chemical Reactions and Equations Science Why should a magnesium ribbon be cleaned before burning in air? Answer Magnesium is very reactive metal. When stored it reacts with oxygen to form a layer magnesium oxide on its surface. This layer of magnesium oxide is quite stable and prevents further reaction of magnesium with oxygen. The magnesium ribbon is cleaned by sand paper to remove this layer so that the underlying metal can be exposed into air. Question
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