Protoplast Isolation Technical & Teaching Notes Apparatus • lettuce (round‚ green lettuce not iceberg) • scalpel or sharp knife • forceps • tile • glass Petri dish • 10 cm3 syringe • 1 cm3 syringe • 13% sorbitol solution • Viscozyme enzyme • Cellulase enzyme • Dropper • small filter funnel • 60 (m gauze square (approximately 12 cm X 12 cm) • tape • centrifuge tube • slide and coverslip A centrifuge‚ high
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Aldous Huxley demonstrates the theme of isolation through foreign and contrasting culture in Brave New World. John‚ “the Savage”‚ is abruptly thrown into a new society that has a government dictated by science and that is far different from his own home. Throughout his turbulent journey in the World State‚ John must maneuver his way through a culture that revolves around science and the perfection of human conditioning‚ and in process he loses everything he holds dear to him that has any semblance
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In Heart Of Darkness the isolation from civilization in the Congo causes people to lose their moral senses. This is quite prominent in Kurtz‚ but Marlow was able to restrain himself from going as far as Kurtz did. This was done by Marlow be observant of Kurtz and his own moral sense. Throughout the story Marlow is able to realize the loss of moral senses in Kurtz. This is due to the isolation Kurtz experienced while he was surrounded by the dense jungle. This led to Kurtz realizing he could do
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The Rye‚ by J.D. Salinger the main character‚ Holden is very secluded and alienated from the rest of the world. He is not alienated in a physical way‚ but in a mental way. Holden is a hypocritical misfit and shows the audience how lost he feels in his life. Holden is lost and confused‚ trying to find his way between two phases in his life. Holden uses alienation as defense mechanism for his self- protection from the outside world‚ and puts up a bitter wall around himself allowing no one to get close
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Despite being surrounded by others‚ many people tend to feel alone in society. This feeling of mental isolation is shown throughout J.D. Salinger’s Catcher in the Rye. A sixteen-year-old boy‚ Holden Caulfield‚ wanders around New York City after being expelled from his most recent school and encounters numerous people‚ particularly women‚ before going back to his family. Though Holden tries to surround himself with others‚ he never seems to be able to connect to them. Holden is constantly trying
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Anne states‚ “Paper has more patience than people‚” (pg.6) confirming her thoughts about the people surrounding her. However‚ the statement could never have been truer for Anne until after entering the Secret Annex. The Secret Annex was a place of isolation where Anne had no one to relate to. In result‚ “Kitty” became her safe haven and her best friend when the conflicts of the Annex became too much to handle. Her mother’s‚
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In The Swan by Charles Baudelaire‚ the speaker suffers from isolation and depression caused by Paris’s rapid urbanization. Rather than openly presenting his strife‚ the speaker acknowledges people and animals who endured suffering‚ isolation and depression. More specifically‚ he acknowledges Andromache and a swan‚ which he encountered during Paris’s transitional period. The poem is organized into flashbacks or reflections which peel back Paris’s modern stature. The speaker relies on Andromache‚ a
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individual can lose all sense of self when denied the basic connections that life provides. In Alice Walker’s “Everyday Use‚” a young woman named Dee struggles to find her foundation. Unfortunately‚ her identity begins to disintegrate as a result of her isolation. Through the utilization of character development‚ Alice Walker illustrates Dee’s distinct inability to connect with the people around her. Dee’s failure to see the value of her family history is the most obvious portrayal of her disconnection.
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the withdrawal of individuals from social interaction‚ through various methods. With the world wide web being ever so convenient in the modern world‚ who would bother to get dressed and walk to the library when everything is sitting there on the web? For once in your life though‚ think of how much you are missing out on social opportunities and the changes the Internet has on your behaviour. The world wide web‚ despite being a giant museum that holds everything from the figure of pi to the pros
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The movie that I have chosen to resemble Erickson’s stage; Intimacy vs. Isolation is Endless Love. In this movie you have a young‚ beautiful‚ shy girl‚ who comes from a wealthy family‚ but throughout her four years of attending Atlanta high school‚ she has not made any friends. Jade‚ has engaged herself in trying to do well in school and wanting to be successful in memory of her brother‚ who passed away because of cancer. David Elliot‚ Jade’s classmate has had a crush on her throughout high school
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