based on the alkaline lysis method of plasmid preparation (extraction at pH 8.0) with slight modifications. Cell lysis reagent sodium dodecyl sulfate is replaced by lipase enzyme present in laundry detergent. A good plasmid preparation can be made‚ which is well suited for subsequent molecular biology applications. By taking safety measures on count‚ contaminants like‚ RNA and protein can be completely avoided with maximized plasmid yield. The resultant plasmid quality and quantity can be well comparable
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Constantinus was Emperor Constantine’s given name. He was born on the 27th of February 285 CE in Naissus‚ Upper Moesia. He was the son of Helena‚ an inn- keeper’s daughter and Constantius Chlorus. His religion was Christianity and he had five siblings‚ three sisters and two brothers. His sisters were Flavia Julia Constantia‚ Anastasia and Eutropia. His brothers were Julius Constantius and Flavius Dalmatius. He died on the 22nd of May 337 CE at the imperial villa at Ankyrona. In 312 CE Emperor Constantine
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In Pliny’s “Letters” to the Emperor Trajan‚ the Roman Empire’s encounters with early Christianity caused Pliny‚ the local political authority‚ to adopt a measured response to the increasingly popular religion‚ instead of instant termination of the immediate threat. This was done in part as an effort to counteract the religion’s spread without antagonizing what was a growing proportion of the population. As Christianity gained traction‚ it became necessary for political administrators to reaffirm
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One would agree that Emperor Qin‚ ruler of China improved his country in many different ways overall. Qin ruled Ancient China during the years (221 BC-207 BC). Qin was a strong and powerful leader. Although the dynasty went down in crisis‚ Qin improved China during his life. The article “Who was Shi Huangdi and what area did he rule?”‚ stated Qin improved China by creating a wall known as The Great Wall of China to protect the civilization. “Shi Huangdi ordered the joining of several earlier walls
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beholder.” This means that everyone defines beauty differently. The dictionary defines beauty as “the quality present in a thing or person that gives intense pleasure or deep satisfaction to the mind.” There have another point about image of beauty which is wealthy. Most of the time people define beauty depending on their culture and usually uniqueness. For example‚ many white people want to their tan‚ and many Asian people want to whiten their skin. When comparing American and Chinese images of beauty
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Dynasty Essay The Qin Dynasty The first Emperor of China. The consequences of his work ensued in the unification of China. He had an ambition for immortality. Further‚ Ying Zheng or better known as Qin Shi Huang was an Emperor like no other; he was truly an epoch-making to China’s culture and history. From 221B.C. to 207B.C. Qin made many contributions to China. He ordered the construction of the Great Wall to protect from the Mongols. This structure took about 2000 years to finish. Moreover
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Taj Hotels‚ Resorts & Palaces CSR Case Study Company Brief The Indian Hotels Company Limited and its subsidiaries are collectively known as Taj Hotels Resorts and Palaces and are recognized as one of Asia’s largest and finest hotel company. Incorporated by the founder of the Tata Group‚ Mr. Jamsetji N. Tata‚ the company opened its first property‚ The Taj Mahal Palace Hotel‚ Bombay in 1903. Today Taj Hotels Resorts and Palaces comprises 108 hotels across destinations in India‚ Maldives‚ Malaysia‚
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INTRO TO NETWORKING NT1210 7/5/2013 CHAPTER 3 KEY TERMS TABLE PG. 154 TCP/IP NETWORK – A COMPUTER NETWORK THAT USES THE STANDARDS DEFINED AND REFERENCED BY THE TCP/IP MODEL. NETWORKING STANDARD – A DOCUMENT THAT DETAILS INFORMATION ABOUT SOME TECHNOLOGY OR PROTOCOL RELATED TO NETWORKING‚ AND THAT DOCUMENT HAS BEEN PASSED THROUGH A REVIEW AND APPROVAL PROCESS THAT CERTIFIES THE DOCUMENT AS A STANDARD. TCP/IP MODEL – AN OPEN COLLECTION OF STANDARDS RELATED TO ALL PARTS OF NETWORKING‚ GROUPED
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Royal World Palace Outer Lands "Haah.." A farmer wiped his sweat off as he was farming the white lotus tuber plants. He finished his daily sowing routine. The farmer looked up at the sky. One with yellow the other with red‚ two suns’ waves were touching his with their might. The weather was hot summer‚ but the harmony of the luminous red and yellow colors in the blue sky reflecting on the greens was worth to look at. The clarity of the view was vivid to remember for a lifetime. "What a
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complex ii. Write it’s structural formula a) Give chemical tests to distinguish [Co Br (NH3) 5] S04 and [Co (NH3)5 SO4] Br 2. Give IVPAC Names of the following i. K2 [ptF6] ii. [Co (NH3)5 (ONO)] CL2 iii. K3 [Co NO2)6] iv. [Cr (en)3 ] CL3 3. Write down the structures of the following Complexes i. Hexamine chromium (III) hexacyano cobaltate (III) ii. Triammine chloroplatinium (II) ammine trichloro palatinate (II) iii. Tris (ethylenediamine)
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