BIOL 1F90 Expt. #1: Protein Quantification Student Name: Carlos Osorio Student ID: 5220710 Lab Section: 34 Date Experiment Performed: Sept. 26th‚ 2012 Lab Partners: K. Cloutier J. Yang ABSTRACT Protein concentration analysis is primarily done through an accepted form commonly referred to as the Bradford Protein Assay. The main purpose of this experiment was to observe and record the various protein samples’ absorbency values through the calibrated readings of a spectrophotometer
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Brief Report on Infant Mortality: A Critical Health Issue Critical Health Issues: HSC 4634 Dewayne Ford Florida A&M University Infant mortality is a significant measure of a country’s overall health status‚ because it encompasses a wide array of factors‚ such as maternal health‚ access to preventive care‚ socioeconomic status‚ and a nation’s public health initiatives. According to the World Health Organization in 2000 the United States was ranked 37th out of 191 countries in terms of overall
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“Denaturation of Proteins” Denaturation is a process in which proteins or nucleic acids lose the tertiary structure and secondary structure which is present in their native state‚ by application of some external stress or compound such as a strong acid or base‚ a concentrated inorganic salt‚ an organic solvent (e.g.‚ alcohol or chloroform)‚ or heat. If proteins in a living cell are denatured‚ this results in disruption of cell activity and possibly cell death. Denatured proteins can exhibit
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Proteins are polymeric chains that are built from monomers called amino acids. All structural and functional properties of proteins derive from the chemical properties of the polypeptide chain. There are four levels of protein structural organization: primary‚ secondary‚ tertiary‚ and quaternary. Primary structure is defined as the linear sequence of amino acids in a polypeptide chain. The secondary structure refers to certain regular geometric figures of the chain. Tertiary structure results from
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Abstract This experiment aimed to study the effect of various denaturants on albumin and casein protein extracts through viscosity measurements. 5 mL samples of native and denatured protein solutions were prepared‚ using -mercaptoethanol‚ urea and SDS as denaturants for albumin‚ and NaOH‚ NaCL‚ HCL‚ -mercaptoethanol‚ urea and SDS for casein. 5 mL blank solutions for each denaturant used were also prepared. The viscosity of the solutions were determined using Ostwald viscometer. ________
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Lab 2: Internet Resources for Protein Biochemistry Part 1-1: A) Protein Accession Number = P00939 Name of Protein: Full Name = Triosephosphate isomerase; Short Name=TIM Organism where the protein is found: Oryctolagus cuniculus = Rabbit Nuber of amino acids in the protein: AA = 248 B) * Amino Acid Sequence: >gi|136066|sp|P00939.1|TPIS_RABIT RecName: Full=Triosephosphate isomerase; Short=TIM;
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Protein function • Chapter 5.1 • Myoglobin: structure‚ O2-binding • Hemoglobin: structure‚ cooperativity in O2binding‚ Hill constant‚ allosteric interactions‚ Bohr Effect‚ BPG-binding and effect • Abnormal Hemoglobins Functions of Proteins Fibrous proteins: collagen‚ keratin‚ silk - give tensile strength‚ shelter‚ protection Globular proteins: • Storage of ions and molecules – myoglobin‚ ferritin • Transport of ions and molecules – hemoglobin‚ serotonin transporter • Defense against pathogens –
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DATE: 11/10/12 COURSE CODE: BIOL 2365 Comparative Biochemistry TITLE: Proteins and Amino Acids RESULTS: Table 1: The results of experiment 1; the Lowry Test Volume of Standard Protein/ Unknown (mL) Absorbance at 750 nm 0 0.000 0.1 0.017 0.3 0.135 0.3 0.155 0.5 0.230 0.7 0.323 0.7 0.310 1.0 0.457 1.0 Unknown 1a 0.463 1.0 Unknown 1b 0.433 1.0 Unknown 2a 0.237 1.0 Unknown 2b 0.159 Table 2: The results of Experiment 2; Ninhydrin Test Amino acid Color X
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Protein Article Research SCI/241 August 1‚ 2013 Dr. Theodore Keneklis Protein Article Research Proteins are made up of amino acids‚ and in our bodies we have proteins that “are part of every cell‚ tissue‚ and organ in our bodies” (Centers for Disease Control and Prevention: Protein‚ http://www.cdc.gov/nutrition/everyone/basics/protein.html). There are complete proteins which are made up from animals. These kinds of proteins are found in meat‚ poultry‚ fish and even cheese. A complete
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Infant Mortality in Milwaukee Stephanie Hawanna PSY 110 Infant mortality is nothing but old news to Milwaukee‚ Wisconsin. It has been said that in the year of 2008‚ there was a rate of 6.9 deaths per 1000 babies that died. This rate is more than counties outside of the United States. In Milwaukee‚ these deaths mostly involves minority groups and families. But what is infant mortality and why is it such a problem? Infant mortality is the measure of infant deaths per year. It is worldwide
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