Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography
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Our words have a strong power. With our speech we have the ability to make worlds or break worlds. The Torah’s laws of speech make it possible for people to live together in peace and build close relationships. One who wants to create a serene home with a pleasant atmosphere should incorporate all the laws of shmiras haloshon into his actions and teach his family to follow suit. We deal with so many people and situations on a daily basis that frustration with others is inevitable. The laws of shmiras
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REPORTIntroductionWater is a liquid that freezes and also melts at 32*F (0*C). Melting and freezing points are temperatures when a solid substance turns to a liquid. Liquid is a substance that flows to fit its container.Water Water is a liquid that is odorless‚ colorless‚ and tasteless. Water freezes and melts at 32*F (0*C). As water freezes it expands by one-eleventh. The amount of pressure on the water when it is freezing changes the melting point. Water that reaches 40*F it is at its maximum density. The molecules
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OIL PURIFICATION A SCIENCE INVESTIGATORY PROJECT CHAPTER I Background of the Study INTRODUCTION Oil used in cooking foods is commonly derived from vegetables. Cooking oil is commonly used for frying and oil used for frying is usually thrown away. The recycling of cooking oil also provides a form of revenue for restaurants‚ which are sometimes compensated by cooking oil recyclers for their used deep fryer oil. Cooking oil recycling also results in less used oil being disposed
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and silver deserve to have their integrity as unique substances respected‚ so do humans understandably try to uphold the purity of their given inclinations and values. Sectarian paths of all kinds come from this need to uphold congruency‚ and our many different schools of thought in both science and religion are the result. The price of purity is truth‚ however‚ for where purity demands homogeneity‚ truth contains multitudes. Whether we seek spiritual transcendence and deny our primitive biology
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Purification of Infliximab[3] begins at Stage 1 with the filtration of the harvest material using a spin filter. The harvest is then clarified and undergoes 3 main chromatographic steps. Firstly‚ at Stage 2‚ the direct protein capture by Protein A affinity chromatography helps to remove a large majority of the impurities whicn include viruses and media components. At Stage 3‚ prior to cation exchange chromatography‚ the purified material in the eluted stream is then subjected to freezing‚ thawing
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and solid C were both extracted from unknown solution by first using chemically active liquid-liquid extract‚ followed by vacuum filtration. Liquid C and solid C were then purified with the use of simple distillation and recrystallization respectively. Through the process of recrystallization‚ the percentage purity of solid C was found to be 6.01%. The melting point range of purified solid C was 117.0 – 119.0C while the boiling point of liquid C was found to be 117C. The identity of solid C and
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ISBN 061549218 ©2011 Copyright Theonosis Publishing LLC The text of this work is dual-licensed under the Open Setting License 1.0 and the Creative Commons Attribute-ShareAlike 3.0 Unported License. For more information see http://creativecommons.org/licenses/by-sa/3.0/ and http://theonosis.com/wiki/index. php?title=Theonosis:Open_Setting_License. The front and back cover are ©2011 Jeremy Thevonot and are dual-licensed under the Open Setting License 1.0 and the Creative Commons Attribute-ShareAlike
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mummion assignment Task 1 – purification Aim We were given a sample of mummion in the form of dried mud from a lake; the aim of the practical was to purify the sample and separate it from the insoluble impurities into the form of mummion salt. Context A long time ago mummion was used to preserve the bodies of ‘mummies’ in ancient Egypt‚ mummion along with other naturally occurring preservatives was used to treat the organs before the bodies were wrapped. This purification practical happens very often
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Ocean County College Department of Chemistry Liquids and Solids Date Submitted: June 21‚ 2015 Date Performed: June 19‚ 2015 Lab Section: Chem-181DL1 Course Instructor: Prof. Amal Bassa Purpose The purpose of this experiment was to determine the boiling point of isopropyl alcohol and determine the melting point of acetamide. Procedure To begin I tied the test tube and thermometer together and inserted an inverted closed-end capillary tube into the test tube. I then
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