NTRODUCTION Often in the study of microbiology‚ the need to identify an unknown microbe may arise. This aids in the recognition of which microbes are harmful or pathogenic as well as those that may be beneficial to us in some way. This report will detail how a similar project was performed in order to identify two unknown micobes using the techniques that were learnt during the course of the semester. We embarked on the project with the knowledge that we would have to identify a Gram(+) as well
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The purpose of Module 11A was to test for the presence or absence of a particular set of functional groups through the use of wet chemical tests. In this manner‚ Unknown A which was a colorless solution‚ was first tested with 2‚4-DNP which after mixing for a few seconds formed a bright yellow precipitate. Although this confirms the presence of either a ketone or aldehyde group‚ one simple chemical test does not completely specify the presence or absence of other functional groups. Therefore‚ a second
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Purpose: The purpose of this experiment was to identify one ketone with Thin Layer Chromatography and one using NMR spectrometry. We will do this by making 2‚ 4 a DNPH derivative and checking the melting points. Theory: By Using specific methods of compounds detection‚ we can match an unknown compound with a known compound because similar compounds will display similar characteristics. In this experiment‚ identifications of the unknown ketone was accomplished using thin layer chromatography
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an Unknown Compound Purpose: The purpose of this experiment was to separate a mixture of two or more unknown compounds and identify them based on their melting point and reactivity with an acid or a base. Compound studied/Reactions: The organic solvent used in this experiment was methyl t-butyl ether (MTBE‚ 2-methoxy-2-methylpropane). The unknown that dissolved in the MTBE‚ but was insoluble in water. MBTE is very volatile and flammable. It was determined in the experiment that the unknown was
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Purpose: The purpose of this was to determine the concentration of sodium carbonate in an unknown sample by titration. The solution of hydrochloric acid was prepared and standardized using Na2CO3. Observations: Week 1: Standardizing hydrochloric acid using sodium carbonate with bromocresol green indicator Table 1: Titration of sodium carbonate using hydrochloric acid Trial | Mass of Na2CO3 (g) | Burette Reading (mL) | Final Volume of HCl (mL) | | | Initial | Final | | 1
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and Report: Introduction: The experiment was conducted to find the unknown molarity of HCL. The unknown molarity of HCL was found by the use of the titration method and then comparing the results between the titrant and analyte HCL solutions. Molarity is a concentration unit‚ it is defined by the number of moles the total volume of the solution. This experiment included a process called titration‚ used to work out the unknown concentration of a known substance. The process of titration is adding
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Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to
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POETRY ANALYSIS THE UNKNOWN CITIZEN BY W. H. AUDEN PENGKAJIAN TEKS SASTRA INGGRIS Fakultas Ilmu Pengetahuan Budaya - Universitas Indonesia Kampus Baru UI‚ Depok The Unknown Citizen By W. H. Auden (To JS/07 M 378 This Marble Monument Is Erected by the State) He was found by the Bureau of Statistics to be (A) One against whom there was no official complaint‚ (B) And all the reports on his conduct agree (A) That‚ in the modern sense of an
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a known quantity of solid dissolved in solution. It may also be used to calculate the molar mass of an unknown acid or base. Purpose: I. Prepare a standard solution of sodium hydroxide II. Standardize a sodium hydroxide solution by using potassium hydrogen phthalate (KHP) III. When given whether the acid is monoprotic‚ diprotic‚ or triprotic ‚ determine the molar mass of the unknown organic acid. IV. Calculate the Molarity of the Soft drink. Materials: * 50 mL Buret
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EXPERIMENT 1 IDENTIFICATION OF UNKNOWN COMPOUNDS Date of experiment : 7th August 2012 Venue: ME204 INTRODUCTION Chemical reactions are regularly categorized into 3 types: oxidation-reduction (redox reaction)‚ precipitation (double displacement) and acid-base reaction (double displacement) Type 1: Oxidation-Reduction Reactions Oxidation-reduction processes include the movements of electrons form oxidants to reductants‚ which lead to increases in oxidation
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