atom or a fluorescent dye is used as label on probe. Reverse Transcription Polymerase Chain Reaction (RT)-PCR Employing PCR-based procedure in analyzing RNA would require cDNA to be synthesized using reverse transcription. When reverse transcription and polymerase chain reaction are combined together‚ it will permit detection of low abundance RNAs. RT-PCR is distinct from Real Time Polymerase Chain Reaction (qPCR). The latter measures the amplification of DNA in quantitative way and it uses fluorescent
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Theory: Esters are derived from carboxylic acids and alcohols with the presence of a catalyst. A carboxylic acid contains the -COOH group‚ and in an ester the hydrogen in this group is replaced by a hydrocarbon group of some kind. Sulfuric acid (H2SO4)is used as a catalyst for this reaction in order to accelerate the rate at which the product is formed. The general formula of an ester is RCOOR’ in which R is the alkyl group that comes from the carboxylic acid while R’ is the alkyl group that comes from
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MEC 325/580 Lab Report CNC Machining Lab Report Date: March 18‚ 2013 Group Leader: Jian Wu Team Members: Group Number: G4 Regment No.: D Insturctor: CONTENTS 1.Objective and introduction. 2.Principles and practice. 3.Design and result. 4.Conclusion and recommendations. Appendices 1. Objective and introduction * Objectives Operate the CNC milling machine using G-codes‚ M-codes in a CNC program. * Equipment: Minitech CNC machine (Series 2) * Machine
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Introduction The study of chemical reactions‚ isomerizations and physical behavior that may occur under the influence of visible and/or ultraviolet light is called Photochemistry. The fundamental principles for understanding photochemical transformations are that light must be absorbed by a compound in order for a photochemical reaction to take place‚ and that for each photon of light absorbed by a chemical system only one molecule is activated for subsequent reaction. This "photo equivalence law" was
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I. Title: Reaction of Metals II. Problem: The purpose of the experiment was to determine if and how different metals react to different solutions. III. Hypothesis: IV. Materials: Dropper‚ Beakers‚ wax pencil‚ Goggles‚ eight test tubes‚ a rack for the tubes‚ three strips of Zinc‚ two strips of Copper‚ three strips of Magnesium‚ steel wool‚ Lead nitrate‚ Silver nitrate‚ Copper sulfate‚ Magnesium chloride‚ Zinc chloride‚ Sodium chloride‚ and Potassium. V. Procedure: In tube 1 add five
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proportional relationship between the square of the period of oscillations to the length of the pendulum.Sources of error for this procedure included precision in both length and time measurement tools‚ reaction time of the stopwatch holder‚ and the accuracy of the stopwatch with respect to the lab atomic clock. The final result of g takes into account the correction for the error introduced using the approximation. There are opportunities to correct for the effects of mass distribution‚ air buoyancy
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isomers of a typical coordination compound. INTRODUCTION: Isomerism played a central role to establish the basic concept in coordination chemistry while reaction kinetics of coordination compound is known as the area of continuing intense research activity. The mechanism of the reactions of coordination compounds relevance to enzyme reactions where the active site is being involve in coordination to a metal ion. PROCEDURE: (A) Preparation of Trans-Dichlorobis(ethylenediamine)cobalt(III) chloride
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energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular reactions. All proteins are made up of amino acids (Johnson‚ 2010). The purpose of this experiment is to determine whether peptide bonds are present in various food substances. The color violet will indicate the presence of proteins. The more intense the color is‚ the greater
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Lab #4: The Immune System Purpose: The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab‚ I will then understand how to perform an ELISA test‚ the purpose an ELISA test‚ and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical
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Acids and Bases Mirza Hasan Mammadov Acids and bases are really important in our daily day life. So let’s start with telling the definitions of them. Starting with acids‚ they are a substance which dissociates in water in order to produce one or more hydrogen ions. Also we can write H+ instead of hydrogen ion. Bases have the same definition‚ although the difference is that it firm hydroxide ions instead of hydrogen ions. We can write OH- instead of hydroxide ion. All alkali is bases but not
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