Contents REAL TIME PCR 2 REAL-TIME QUANTITATIVE PCR (qPCR) 2 BASIC PRINCIPLE 3 TYPES OF PCR 4 qPCR STEPS 4 ONE-STEP OR TWO-STEP REACTION 6 Overview of qPCR and qRT-PCR components 6 REAL TIME PCR SYSTEM: 7 SOFTWARES FOR DATA ANALYSIS AND PRIMER DESIGNING 8 STANDARD REAL-TIME PCR PROTOCOL 9 ASSAY DESIGN 9 2. Nucleic acid purification 9 3. Reverse transcription 9 4. Controls and normalization 9 5. Standard curve evaluation of efficiency‚ sensitivity‚ and reproducibility
Premium Polymerase chain reaction
ELECTROPHILIC ADDITION PURPOSE The purpose of this lab is to learn how to synthesize 2-methyl-2-butene into 2-bromo-2-methylbutane using addition of hydrogen bromide. THEORY Chemical Compound Molecular Formula Molecular Weight Boiling Point Melting Point Density Amylene CH3CH=C(CH3)2 70.13 g/mol 36 oC -134 oC 0.662 g/ml Hydrobromic Acid HBr 80.91 g/mol 126 oC -11 oC 1.490 g/ml 2-Bromo-2-MethylButane C5H11Br 151.05 g/mol 107 oC 1.18 g/ml Sodium bicarbonate NaHCO3 84.007 g/mol Decomposes
Premium Chemistry Chemical reaction Hydrogen
SCI235 – Health and Fitness Reaction paper – Trust between parents and their children Discussing the example with the stepmother going through her stepson’s phone made me think a lot about where the line between respecting your children‚ but at the same time looking out for them is drawn. Growing up my parents has had very different ways to go about this line. My dad has always been very clear with rules‚ and what’s wrong and right and such‚ but he also left a lot of space for us to experience
Premium Family Father Mother
PE13 B25 February 2 2012 REACTION PAPER “MAPUA CARDINALS VS. EX CARDINALS” It was an exciting day because of the basketball game held last January 28 2012 in Mapua’s gymnasium. The game was in accordance with the commemoration of the founding anniversary of the institute. It was a fight between the present Mapua basketball players and the ex-cardinals. It was a game between yellow team and red team. The yellow team was composed of Madrid‚ Palomique‚ Espinosa‚ Cinco‚ Alcantara‚ and Sta
Premium Play Basketball Game
Reaction on Completed Staff Work Many in different organization‚ plans and programs are usually formulated and implemented by top officials‚ and problems encountered were given solutions also. These are the common scenario wherein the burden is placed on one’s shoulder only. Through the implementation and adoption of CSW in an organization‚ these will relieve stress and anxiety to any top officials. The common scenario in many organizations‚ subordinates bring their managers a problem and sometimes
Premium Government Management Separation of powers
Colin Burlison 1/9/13 AP European History Thermidorian Reaction DBQ In the time of the National Convention‚ (a governmental body existent for three years of the French Revolution) the government desired for total change from the Old Regime. The final change stemming from this desire was the introduction of the French Republican Calendar. In 1789‚ the Cahier de doléances (report of grievances)‚ from the Third Estate of Château-Thierry‚ said that they asked for the number of religious
Premium French Revolution National Convention
REACTION PAPER (ON-PREMISE CATERING) During our on- premise‚ I’ve learned a lot of things like how to prepare that kind of event‚ how to set up‚ how to prepare everything. And to become success the event we must cooperate‚ be unite so that would finish as soon as possible‚ that day were so busy‚ each everyone of us was working‚ but there are some times who are not working‚ of course we can’t avoid that‚ but we are trying our best 2 finish it. There program was started‚ everyone and everything was
Premium Dinner Meal The Help
INSIDE JOB | TRAJANO‚ ALMIRA L. BSA803 | | Reaction Paper | This crisis‚ the United States and the world is facing is the effect of the lack of control and regulation on certain financial activities. Because of the continuing opposition of different individuals and companies with the imposition of regulations on these financial activities‚ it continued to be uncontrolled under the administration of different Presidents of the United States. Due to advances in technology‚ many
Premium Debt Finance
11 April 2013 Profesor Maria Duarte Reaction Paper 3.The national agenda of Porfirio Díaz oriented Mexican economy toward exportation and growth‚ how did Díaz’s modernizing agenda affected elite and lower-classes in the process? Porfirio Diaz was known as a ruthless revolutionary who revolutionized Mexico first by gaining presidency by paying off his opposition and allies along with war. Diaz system failed in the end because he failed to build an enduring system that could have outlived
Premium Mexico Wage Porfirio Díaz
Jane Doe December 6‚ 2012 Post-Freudian Psychoanalytic Theory of Adam and Eve The story of Adam and Eve is one of the most culturally important and known stories in the Bible regarding the origin of mankind. It’s generally followed by Judeo-Christians but is also grasped by other religious views‚ though many tend to overlook minor key details that may alter the whole interpretation. First‚ God created a man named Adam to primarily tend to the garden he planted in Eden. There were many trees
Premium Garden of Eden Adam and Eve Serpent