EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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DNA is the common name for Deoxyribonucleic acid. This is made up of nucleic acids containing deoxyribose (sugar)‚ consisting of complex molecules‚ present in the chromosomes of all plant and animal cells‚ and carrying in coded form instructions for passing on hereditary characteristics. The DNA molecule takes the shape of a double helix‚ a simple structure that resembles gently twisted ladders. The rails of the ladder are made of pairs of nitrogen-containing nucleotides‚ which are subdivisions
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plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal DNA from your
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Comparison chart DNA RNA Stands for: DeoxyriboNucleicAcid RiboNucleicAcid Definition: A nucleic acid that contains the genetic instructions used in the development and functioning of all modern living organisms (scientists believe that RNA may have been the main genetic material in primitive life forms). A single-stranded chain of alternating phosphate and ribose units with the bases Adenine‚ Guanine‚ Cytosine‚ and Uracil bonded to the ribose. RNA molecules are involved in protein synthesis
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Digital Plagiarism: The Role of Society and Technology • [pic] • [pic] Ryan Kennedy Examines the application of the World Wide Web in class education and research and the ways in which the Internet has enabled cheating and given educators ways to fight plagiarism. Internet-based information has had a profound effect on the way people can now educate themselves from a pool of seemingly endless content. It is estimated by the NEC Research Institute that there are more then 1.4 billion
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The DNA double helix model In 1953 James Watson and Francis Crick made a scientific breakthrough when they discovered the structure of DNA. From this structure they inferred that DNA contains information written in a genetic code‚ is passed from one generation to the next. This discovery was to be a key step in the genetics revolution DNA has four molecular building blocks called nucleotides; adenine (A)‚ thymine (T) guanine (G) and cytosine (C). Purine base always pairs (by hydrogen bonding) with
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Homework # 1 DNA 2. Name the four differences between RNA and DNA. 1) DNA is double stranded‚ RNA is single 2) DNA has Adenine‚ Guanine‚ Thiame‚ and Cytosine as nitro bases. With RNA‚ anywhere you would expect to see Thaime‚ you see Uracil instead. 3) DNA has no types‚ RNA has 3 types: mRNA‚ rRNA‚ and tRNA. 4) DNA and RNA have different sugars. 3. For the following DNS strand‚ provide the replicated strand : GATTACACATTAG CTAATGTGTAATC 4. For the following DNA sequence provide
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appeared to have a cord around her neck. While assuming the role of a responding investigator‚ I would apply the three basic elements of an investigation. The first element is searching the crime scene. The search of the crime scene is to look for any clues and for evidence that may like to the crime. These object or material may be anything that may serve as tool by witch I can use to give a good idea of what may happen during the crime. The next element is to Collecting and preserving evidence
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DNA STRUCTURE AND FUNCTION QUIZ Provide answers to each of the following. You may consult your text and fellow students. Each numbered item counts 2 points. 1. If one strand of a DNA double helix has the sequence GTCCAT‚ what is the sequence of the other strand? a. ACTTGC b. CUGGTU c. CAGGTA d. CAGGUA e. TGAACG 2. If a strand of DNA has the sequence AAGCTC‚ transcription will result in a(n) ______. a. single DNA strand with the sequence TTCGAG
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DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation‚ scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites‚ ensuring that all DNA fragments that contain a particular sequence have the
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