Introduction The purpose of this lab is to determine which pigments in a plant support or effect photosynthesis‚ based on starch production‚ which wavelengths of light are involved in photosynthesis‚ and identify plant pigments found in a plant leaf by means of paper chromatography. Life on Earth is dependent entirely on the energy from the Sun‚ not only to keep the planet at a suitable temperature but also to provide the energy required to sustain life. The energy of the Sun‚ in the form of
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LABORATORY REPORT FOR BIO411 Experiment 5 : Cellular Respiration Experiment 6 : Photosynthesis Title: Cellular Respiration Objective: To observe and determine cellular respiration in yeast/onion cells. * Measure respiration rate using different substrates. * Measure respiration rate at different temperature. Introduction: In this laboratory experiment‚ we are given 3 task. The first one is respiration in yeast. Second is respiratory indicator and the third one is observing
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2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure: A.spotting
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oleracea and Musa acuminata‚ which use different wavelengths of light to display various pigments of color. The light energy for photosynthesis calls for an array of light to be absorbed by the plants. In this experiment‚ the different wavelengths of light can be traced and graphed by extracting chlorophyll from these two plants and placing them into a spectrophotometer‚ which identifies what wavelengths of light a pigment absorbs. It was found that Musa acuminata‚ commonly referred to as banana palm‚ showed
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AP Biology Lab Four: Plant Pigments and Photosynthesis Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene‚ the most abundant carotene in plants‚ is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found
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Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
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To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex mixtures. Using this technique‚ the course of a reaction
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TABLE OF CONTENTS S no Topic Page number 1 Introduction 1 2 Objective 4 3 Scope and limitations 5 4 Theory 6 5 Experiment 11 6 Procedure 13 7 Observations 17 8 Result 18 9 Bibliography 19 INTRODUCTION Chlorophyll is a green photosynthetic pigment found in chloroplasts of organisms like cyanobacteria‚ algae and plants. Its name is derived from the Greek words chloros‚ meaning ‘green’ and phyllon meaning ‘leaf’. First isolated
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- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Spectrophotometer Lab Introduction: A spectrophotometer is an instrument that measures the quantitative amounts of light of different wavelengths absorbed and transmitted by a pigment solution (Mitchell‚ Reece). The spectrophotometer includes a light bulb‚ a reflector‚ and a detector. When a sample is in place and the chamber lid is closed‚ light from the light bulb passes through it. The detector measures the amount of light
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Milton Cano Chauca Anthocyanin in spinach vine (Basella rubra) fruits Experimental t-test In conclusion‚ the pigment extracted from the fruits of spinach vine (B. rubra) was more stable at pH 5 and pH 6. Increases in temperature and the presence of light affected the stability of this pigment. Results demonstrate that this pigment exhibits good stability and could be applied to foods as a natural food color. The study is limited to the stability of anthocyanin in spinach vine (Basella rubra) fruits as
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