3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
Premium Molecular biology Western blot Southern blot
Southern Pulp and Paper Introduction In this work I am going to identify the Southern Pulp and Paper’s Toccoa mill problems and issues that are available to mill’s manager Curtis Shelton. Part 1 In considering the overall process on the pulp and bleached products industry it is always good to start with the main performance objectives and measures. One of the first important issues is the process design and layout. How the process architecture looks like and how well it is designed
Premium Process management Business process Business process reengineering
there were several colonies but the ones that stood out the most were the New England Colonies and the Virginia colony. There were many differences‚ for example‚ New England colonies were full of families while the Virginia colony was mostly dominated by males. They mostly had differences and had few things in common. The foundations of the colonies were different. The New England colonies were founded because the founders wanted freedom of religion. The founders of these colonies were the Puritans
Premium Slavery in the United States Slavery Thirteen Colonies
An Examination of Southern Dialect as Seen in the Works of William Faulkner In the writings of William Faulkner‚ the reader may sense that the author has created an entire world‚ which directly reflects his own personal experience. Faulkner writes about the area in and around Mississippi‚ where he is from‚ during the post-Civil War period. It is most frequently Northern Mississippi that Faulkner uses for his literary territory‚ changing Oxford to "Jefferson" and Lafayette County to "Yoknapatawpha
Premium English language American English African American Vernacular English
The Virginia Colony vs. The Massachusetts Bay Colony The Virginia Colony and the Massachusetts Bay Colony were both similar and different on three main topics: religion‚ economics‚ and demographics. Religious views and importance differentiated greatly between the two colonies. New Englanders‚ the area in which the Massachusetts Bay Colony settled‚ came to America to exercise religious beliefs that were not allowed before the English Civil War and after the Restoration. They were made up of
Premium Massachusetts Massachusetts Bay Colony United States
China Southern Airlines was founded in 1995 and is based in Guangzhou. It is China’s largest airline in terms of fleet size‚ with 309 aircraft. The airline employees over 45‚500 full time employees. Its main hubs include Guangzhou and Beijing‚ but it operates in over 150 cities throughout China. Furthermore‚ China Southern operates 616 domestic 82 routes‚ including 90 international routes and 25 routes in Hong Kong and Macau. As China’s only company that is in world top ten passenger airlines
Premium Lufthansa Delta Air Lines Cathay Pacific
Comparison between the Colonies of Chesapeake and New England This essay will be analyzing and comparing & contrasting the colonies of Chesapeake and New England. This paper’s main concern is how these colonies are so dramatically different and what aspects of the colonies make them so. This paper will argue considerable differences in settling and motives to settle had a dramatic effect on the initial success of the colonies. Chesapeake had a tremendous death rate of 65-percent of their original
Premium Thirteen Colonies Human migration Government
and Spanish colonies were able to flourish in the new world even though they differed in motives for colonization and social layout; yet both colonies were similar to each other in the fact that they had common economies and like ways of treating the indigenous population. Once they established land in the new world‚ each country was able to find a new source of wealth‚ either from precious metals or from building necessities such as lumber. Seemingly‚ the British and Spanish colonies were some of
Premium Colonialism
means to have a Southern accent‚ your mind goes straight to the stereotypical renditions you have heard from media portrayals of the South. Probably‚ the examples you think of are highly exaggerated‚ like Scarlett O’Hara’s Deep South‚ r-less rendition of a Southerner exclaiming Well ah nevah! (Bailey 11) or like those in “Gone with the Wind” or “Dukes of Hazzard County‚” both of which reinforce the negatively stigmatized‚ stereotypical Southern accent (Lippi-Green 218). Southern American English
Premium English language American English Southern United States
In the above passage‚ it represents how in the 1700s the Scottish-Irish immigrants came to America broke but were culturally competent with literacy unlike many of the poor Irish Catholic immigrants that arrived in America due to the potato famine in Ireland. The change over time for the Scots-Irish immigrants began with a culturally diverse and economically inferior populous during the eighteen century facing social and religious stigmas connected to Protestantism which differed from most other
Premium United States Ireland Immigration to the United States