EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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Throughout the Namesake‚ Jhumpa Lahiri uses both diction and the theme of isolation to show how Ashima and Ashoke constantly oppose Gogol and his beliefs because of the different cultures in which they grew up. After moving to the United States‚ Ashima and Ashoke possess an extreme sense of seclusion because they must raise a son in a foreign country that they are not familiar with. While Ashima lies in the hospital bed waiting to give birth to Gogol‚ she immediately feels isolated. Without Ashoke
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“Social isolation is one of the most devastating things you can do to a human being; I don’t care how old you are.”-Rosalind Wiseman. The role of isolation in society is quite similar to its purpose in literature‚ it creates an internal struggle. Isolation is often used in literature to portray a feeling of emptiness which furthers the plot. Ernest Hemingway‚ author of modern short stories‚ uses the theme of isolation in his literary works to express feelings of unhappiness or uncertainty. “Cat in
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The American Literature‚ as in any other country was based on the phase and situation where the country was going through at that moment. The 20th century‚ has an indelible trail on it‚ left by the World War I (7/28/1914 – 11/11/1918)‚ this was a turning point to the American Literature‚ which was molding its shape before this event‚ and continued to do it subsequently. Significant movements continued to develop the authors in the faces of literature such as drama‚ poetry‚ fiction and criticism.
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Loneliness and Isolation in Jane Eyre In Charlotte Bronte’s novel “Jane Eyre”‚ the eponymous protagonist suffers throughout the plot from loneliness and isolation‚ and these two themes interweave for the duration. Jane’s loneliness and isolation are repeatedly linked to her “physical inferiority”‚ and this phrase is used by Jane to describe herself very early on in the novel. Jane is small‚ underdeveloped‚ pale and timid‚ which often means she finds herself helpless and different to everybody
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The Isolation‚ purification and identification of Proteins assayed From Bovine Liver Using SDS Gel Electrophoresis‚ Mass Spectroscopy and Western Blotting Abstract The purpose of the experiment was to isolate and recognize varying protein solubilization and assaying methods by using bovine liver protein. The experiment implicated the impact of different types of solvents like ethanol‚ water‚ PBS‚ PBS+1% Triton x-100‚ and PBS+2% SDS on protein solubilization. Bradford and Ghosh/Dumbroff
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What is Literature? Language Department IPGK Pendidikan Teknik Based on your experience learning literature‚ what is literature? What are the characteristics of literature? Do you need to learn literature? • Traditionally‚ literature is “imaginative” writing. • However‚ the distinction between “real” and “fake” or “fact” and “fiction” isn’t always a good distinction; many classical works were non-fiction. Literature is Subjective • Since the 1980’s‚ the “literary
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Miguel De Cervantes The son of a deaf surgeon‚ Miguel de Cervantes was born near Madrid in 1547. He became a soldier in 1570 and was badly wounded in the Battle of Lepanto. Captured by the Turks in 1575‚ de Cervantes spent five years in prison. He was freed in 1580 and returned home. De Cervantes finally achieved literary success in his later years‚ publishing the first part of Don Quixote in 1605. He died in 1616. For nearly his entire life‚ Miguel de Cervantes struggled financially. His father
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other churches. Gothic literature began in England with the novel the ‘Castle of Oranto’ by Horace Walpole. From this novel‚ gothic fiction developed and flourished‚ becoming a significant literary genre that inspired famous works such as Frankenstein‚ Dracula and Dr. Jekyll and Mr. Hyde. Gothic texts share the central theme of horror‚ and incorporate elements of romanticism to create a dark‚ mysterious atmosphere and evoke feelings of fear within the reader. Gothic literature is‚ in essence‚ a genre
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The literature of early New England reflected the lives and beliefs of Puritan colonists. The basis of their society was structured strictly towards humility and the worship of god. Every action the puritans committed reflected an attitude of humility‚ for they feared gaudiness would offend the glory of god. As such‚ the everyday behaviors of puritans were basic and simple‚ from the food they ate to the clothes they wore. It is no surprise then‚ that the Puritan mind-set of depravity would also shine
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