chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part of the experiment dealt with breaking down the spinach leaves in a mortar and pestle. Acetone was
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support‚ silica gel‚ which acts as a Lewis acid catalyst to facilitate the reaction. The reaction forms a porphyrinogen‚ which is then oxidized to the porphyrin product by atmospheric oxygen. Column chromatography is performed for the isolation and purification of tetraphenlyporphin‚ and the thin layer chromatography for analysis.It was concluded that the renention factor(Rf) of the 5‚10‚15‚20- Tetraphenylporphyrin with a percent yield of 61% Introduction: In this experiment 5‚10‚15‚20‚ tetraphenylporphyrin
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Introduction: This experiment involves an extraction of a natural product using the techniques of steam distillation. The principle component of oil of cloves is an aromatic compound‚ which is identified by thin layer chromatography (TLC). Eugenol is widely used in dentistry‚ due to its analgesic‚ antiseptic balsamic qualities. It is ideal for curative for pulp hyperemia (the soft‚ sensitive substance underneath the enamel and dentine of a tooth) and pulp its. In dentistry eugenol is also used to
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Separating Substances: Identifying Food Dyes with TLC Background The color of food is an integral part of our culture and enjoyment of life. Who would deny the mouth-watering appeal of a deep-pink strawberry ice cream on a hot summer’s day or a golden Thanksgiving turkey garnished with fresh green parsley? Even early civilizations such as the Romans recognized that people "eat with their eyes" as well as their palates. Saffron and other spices were often used to provide a rich yellow color
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The purpose of column chromatography is to separate one compound from another by the differing polarities in the compound. This technique is important in organic chemistry because certain experiments may require the separation of compounds in solution to be used for chemical synthesis and/or analysis of a particular desired product by isolating it. Fluorene is to be separated from 9-fluorenone using the technique of column chromatography. The success of separation and purity is determined with analyzation
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Stereochemistry of Dihydroxylation Reactions Question: Can the stereochemical outcome of dihydroxylation reactions be determined by thin layer chromatography? Overview: In this experiment‚ you will perform two reactions that transform alkenes into diols. While similar in functional group transformation‚ these reactions may lead to stereochemically different products. The two possible products are diastereomers of one another‚ and therefore have different physical chracteristics. After
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CODEINE AND PARACETAMOLPRACTICAL REPORT ON THE ISOLATION AND IDENTIFICATION OF CODEINE AND PARACETAMOL AIM: To extract codeine and paracetamol from its tablet by solvent extraction and tentatively identify in comparison to standards using Thin Layer Chromatography. INTRODUCTION: Codeine or methyl morphine‚ an alkaloid‚ was first isolated in 1832 from raw opium. It concentration ranges from 0.2% to 0.8%. Mostly used for its analgesic‚ anti-tussive and anti-diarrheal capabilities (Tremlett‚ Anderson
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presence of 95% ethanol producing long red needles. 0.536g‚ 2.07mmol‚ 158-160°C (160°C lit3)‚ 54.6% recovery. Thin layer chromatography was performed in three different eluents‚ hexane‚ toluene‚ and acetone. The chromatography compared aniline‚ 2‚ 4-dinitrobromobenzene‚ and a sample from the mixture of the two reactants when the reaction first began to take place. Another thin layer chromatography used a product sample an hour after the reaction first began and the eluent that showed the best separation
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ANALYTICAL METHODS FOR FUNCTIONAL FOODS & NUTRACEUTICALS LABORATORY REPORT LAB 2: FAT & OIL FROM OIL-SEEDS: PART II THIN LAYER CHROMATOGRAPHY ANALYSIS (TLC) COORDINATORS: ASSOC. PROF. DR. JAMALUDDIN BIN MOHD DAUD ABDUL AZIM ADNAN 1016741 INTRODUCTION TLC chromatography or thin layer chromatography is a type of planar chromatography. TLC is routinely used by researcher in the field of phyto-chemicals‚ biochemistry etc. to identify the components in a compound
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There can be both advantages and disadvantages in using a TLC sheet of a different length. For example‚ it becomes a disadvantage if the TLC sheet in this lab was 5 cm shorter. If this were the case‚ then (Sample A) would not have shown up or the second spot of unknown substance #3. Moreover‚ the unknown substance‚ which consists of (Sample A‚ would be incorrectly identified as a pure substance and/or as consisting of only (Sample D). Contrary to using a shorter TLC sheet‚ a longer TLC sheet might
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