3.6. Conclusion Various solvent extracts from mahua flower and fruits showed varying degrees of antioxidant activity in different test systems in a dose-dependent manner. The antioxidant activity was correlated with the amount of total phenolics present in the respective extracts in each assay. 50% acetone proved to be the most efficient solvent for extraction of antioxidants from mahua as the related extract contained the highest amount of phenolic compounds and also exhibited the strongest antioxidant
Premium Protein Solvent Oxygen
Experiment 9 Thin Layer Chromatography Experiment: 9 Title: Thin Layer Chromatography Introduction: Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture)‚ however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis. Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of
Premium Thin layer chromatography Chromatography
Organic Chemistry Laboratory I Experiment #6 - Column and Thin-Layer Chromatography (TLC) of a Spinach Extract Pre-Lab Assignment (1) List 5 ways that TLC can be used in an organic chemistry lab experiment. To identify an unknown‚ to monitor the course of a reaction and assess the purity of its product‚ to determine the best solvent for a column chromatography separation‚ to determine the somposition of each fraction from a column chromatography separation‚ and to determine whether a substance purified
Premium Chromatography Thin layer chromatography Chemistry
slowly to induce crystal growth 3. filter resulting mixture to isolate crystals Reading: Mohrig‚ Hammond & Schatz Ch. 15 pgs 183-197 Ch. 10 pgs 104-113 Ch. 14 pgs 174-182 • Scale: 5-10 mg discovery based research - a new material prepared in a lab 1‚000 kg + commercial applications - sugar refining‚ synthesis of pharmaceutical agents‚ etc. Recrystallization Recrystallization • Molecular selection - based on size‚ shape‚ & functionality • Molecular selection - based on size‚ shape
Premium Thin layer chromatography Crystal Melting point
COLUMN AND THIN LAYER CHROMATOGRAPHY Mark Paul P. Pastrana‚ Mariah Ericka M. Patawaran‚ Princess Juneire M. Peligro‚ Francisco Q. Pua III‚ Rose Anne L. Quyo and Janille P. Ragpa Group 8 2B Medical Technology Organic Chemistry Laboratory ABSTRACT The main objectives were to separate the colored components of malunggay leaves by means of column chromatography‚ as well as to determine the purity of the components using thin layer chromatography (TLC) and measure the Rf values of the colored
Premium Thin layer chromatography Chromatography
the lab was to separate and analyse analgesic drugs in a drug tablet. The method used to separate the components was Thin Layer Chromatography (TLC) with silica adsorbent as the stationary phase and 0.5% glacial acetic as the mobile phase. In one plate‚ five known samples were used as the reference‚ that is: Aspirin; Caffeine; Ibuprofen; and Salicylamide. Aspirin and Salicylamide were the only samples that fluoresced. On a second plate‚ the tablet sample was developed. The results of the lab showed
Premium Thin layer chromatography Chromatography Aspirin
IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different solubilities
Free Chromatography Thin layer chromatography
described by Ault1 and the lab manual2. 2‚4-dinitrobromobenzene (0.92g‚ 3.75mmol‚ pale yellow solid) and aniline (0.7mL‚ 0.7g‚ 7.5 mmol) were boiled in ethanol (10mL) under reflux until all solid dissolved. After the mixture cooled to room temperature‚ short‚ red needles were collected by suction filtration. The product was then recrystallized in the presence of 95% ethanol producing long red needles. 0.536g‚ 2.07mmol‚ 158-160°C (160°C lit3)‚ 54.6% recovery. Thin layer chromatography was performed in three
Premium Thin layer chromatography Chromatography
liquid-liquid extraction. Then crude material obtained was then purified by sublimation. The isolated caffeine was subjected to thin layer chromatography (TLC) together with a standard caffeine. The Rf value obtained for both isolated caffeine and standard caffeine was 0.24. Keywords: solid-liquid extraction‚ multiple liquid-liquid extraction‚ sublimation‚ thin layer chromatography‚ percent caffeine Introduction Many organic compounds are obtained from natural sources through isolation. In this
Premium Tea Solvent Caffeine
SCIENCE SBT 4534 ANALYTICAL METHODS FOR FUNCTIONAL FOODS & NUTRACEUTICALS LABORATORY REPORT LAB 2: FAT & OIL FROM OIL-SEEDS: PART II THIN LAYER CHROMATOGRAPHY ANALYSIS (TLC) COORDINATORS: ASSOC. PROF. DR. JAMALUDDIN BIN MOHD DAUD ABDUL AZIM ADNAN 1016741 INTRODUCTION TLC chromatography or thin layer chromatography is a type of planar chromatography. TLC is routinely used by researcher in the field of phyto-chemicals‚ biochemistry etc. to identify the
Free Chromatography Thin layer chromatography Solvent