Gabriel Alizaidy Liquid Chromatography September 14‚ 2009 Objective: To separate the components of unsweetened‚ grape flavored Kool-Aid. Procedure: Using two syringes‚ inject different concentrations of alcohol to extract red and blue dyes‚ and artificial flavoring. Data: Conclusion: Different dyes are shown when separated by different concentrations of isopropyl alcohol. Discussion of Theory: Chromatography‚ resolution and selectivity played major roles in making the experiment work
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In paper chromatography‚ some compounds in a mixture travel almost as far as the solvent does; some stay much closer to the base line. The distance travelled relative to the solvent is a constant for a particular compound as long as you keep everything else constant. Some compounds may travel farther than others because they may be composed of more components‚ thus traveling farther. For example‚ black is composed of every color of the rainbow‚ while yellow is composed of less colors. As a result
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Experiment: The intention of the experiment is to use solvent extraction methods to separate a mixture containing a carboxylic acid and a neutral compound. Once recovered‚ the solids were purified by recrystallization and examined by thin-layer chromatography‚ and their identities were derived by melting point and infrared (IR) spectroscopy. Extraction is a procedure that selectively dissolves one or more of the mixture compounds into a proper solvent. Extraction refers to the moving of compounds
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experiment is called chromatography. The materials used consisted of – 3 pieces of chromatography paper‚ a tall clear glass‚ water‚ table salt‚ 1 tsp. measuring spoon‚ an 8 oz. measuring cup‚ pencil‚ ruler‚ 6 washable markers‚ and paper towels. The markers used in this experiment have to be water soluble therefore I used washable markers made by Crayola. The colors I chose were brown‚ teal‚ red‚ orange‚ black‚ and green. The salt solution served as the mobile phase while the chromatography paper served
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Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
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The purpose of column chromatography is to separate one compound from another by the differing polarities in the compound. This technique is important in organic chemistry because certain experiments may require the separation of compounds in solution to be used for chemical synthesis and/or analysis of a particular desired product by isolating it. Fluorene is to be separated from 9-fluorenone using the technique of column chromatography. The success of separation and purity is determined with analyzation
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Thin Lenses Group members: Trevor Kitts Ryan Mutsch Author: Trevor Kitts 1402L September 10‚ 2012 Introduction In this week’s lab we looked at the movement of light between multiple lenses and their focal points. Procedure List of materials: Optics Bench‚ equipped with laser and triangular like prism. First‚ we aligned the laser with the 0⁰ mark and the center of the prism so that the light hit the bottom of the prism in a perpendicular form. Second‚ we observed that the light
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Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy
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In this Laboratory experiment‚ my lab partner Alexander and I were able to understand thoroughly the physical and chemical properties of salt (NaCl) and sand (SiO2). Followed by the right procedure we were able to design and test out the components of both NaCl and SiO2 in order to separate the unknown mixture that we were able to find out about. We were also given the task to provide the percent composition of the mixtures. Therefore‚ the separation of components among this experiment allowed us
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