from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The polar absorbent material in the column separated the different pigments by allowing the least polar molecules to travel through the column faster than the more polar molecules. The different pigment layers were collected‚ dried‚ and
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blue and red wavelengths have more absorbance‚ the green doesn’t have a high absorbance. This lab is useful to help demonstrate the existence of various leaf pigments by using the process of paper chromatography.
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Back Laboratory Program SAFETY IN THE CHEMISTRY LABORATORY 786 Pre-Lab Extraction and Filtration 790 1-1 Mixture Separation 792 1-2 Water Purification 794 3-1 Conservation of Mass 798 4-1 Flame Tests 801 Pre-Lab Gravimetric Analysis 828 13-1 Separation of Pen Inks by Paper Chromatography 830 13-2 Colorimetry and Molarity 834 14-1 Testing Water 838 804 7-1 Separation of Salts by Fractional Crystallization 806 7-2 Naming Ionic
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Ultra Thin Cloak Can Match Any Background! In the Movie Predator there was an Alien that would uses a Cloaking Device that would hide the Alien in Plain Sight‚ But the effect is far from Perfect‚ due to its attempt to conceal itself is Thwarted from the distortion of Light bending around it. Now‚ Researchers built an Ultrathin “Invisibility Cloak” that Gets around the Problem of Light Bending by turning objects into Flat‚ Perfect mirrors. Invisibility Cloaks are Designed to bend light around an
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called Josh. They aren’t especially close‚ but she often wishes that they were and sometimes pretends that they are. Josh looks a lot like her – both have dark brown hair and fair skin‚ but his eyes are darker. She often struggles with the idea of being thin‚ but she doesn’t understand why. She doesn’t play quidditch‚ but she gets jealous of people
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Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012 Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography‚ determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-
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Lab: investigating hooked law with springs Purpose: to find spring constants of different springs using the slope of a graph of change in heights vs. the weight force. Also‚ to be able to understand how spring constants change when you add springs in a series or paralle Pre lab predictions: We predicted that the graph of gravitational force (mg) as a function of stretch (delta x) would look like Data: Spring #1: y = 8.2941x + 0.0685 This table represents the different distances that
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Paper chromatography is a useful technique for separating and identifying pigments and other molecules from cell extracts that contain a complex mixture of molecules. The solvent moves up the paper by capillary action‚ which occurs as a result of the attraction of solvent molecules to the paper and the attraction of solvent molecules to one another. As the solvent moves up the paper‚ it carries along any substances dissolved in it. The pigments are carried along at different rates because they
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The goal of this lab was to create and purify DHFR from BL21 ecoli using column chromatography. To know if we isolate DHFR their will be one band at 53 KD or above 50 KD and in our desalted eluate page should have the highestOD₂₈₀ which translates to the highest enzyme activity .Column chromatography is a method used to purify individual chemical compounds from mixtures of compounds. The main advantage of column chromatography is the relatively low-cost and disposability of the stationary phase used
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combination of salicylic acid and acetic anhydride.The goal of this experiment was to determined the purity of aspirin using different methods. These methods included‚ reacting salicylic acid with FeCl3‚ finding its melting points‚ and by doing Thin-layer chromatography. When you reacted salicylic acid with FeCl3‚ changes in color will occur. Pure Aspirin has a melting point around 135°C and a pure salicylic acid has 158 °C. TLC is used to separate compounds based on affinity for stationary phase or mobile
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