Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing
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Information and Research 1. Chromatography is an analytical methor or technique that serves mainly as a tool for the examination‚ separating and identifying mixtures of chemical substances that are or can be coloured. 2.check the presence of any contamination in the manufactured compounds h as medicine‚ Contaminants in rainwater Analysis of narcotics Detection of substances in urine http://en.wikipedia.org/wiki/Paper_chromatography http://www.chemguide.co.uk/analysis/chromatography/paper.html http://chem
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Mobile liquid phase is always passed stationary phase during chromatography. Developed TLC plate is then observed under short wave ultraviolet light‚ where most TLC plates contain fluorescent mixed with silica gel that allow to glow green color. Compounds will absorb the UV light and appear as dark spot against the green color TLC plate. In a column chromatography‚ cotton and sand is placed in purpose of holding silica gel and prevent leakage of adsorbent particles
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Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation‚ you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf‚ which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment.
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4. The Medium Access Sub Layer Broadcast channels [or multi-access channels] are a category of networks and the key issue is how to determine who gets to use the channel when there is competition for it. The protocols which define these factors belong to a sub layer of data link layer called the MAC(medium access control)sub layer. ALOHA: Norman Abramson devised a new and elegant method to solve the channel allocation problem called the “ALOHA” system which used ground-based Radio broad
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC).
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the right numbers for our calculations. Mixture is defined as a substance made by mixing other substances together‚ in this case we will be dealing with salt and sand of course to undergo the process of separation. Distillation‚ Filtration‚ and Chromatography are known alternatives to separate the
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Post Lab #4- Column Chromatography Organic Chem 3418-2 March 3‚ 2011 Theoretical Background- The fluorene and fluorenone mixture was separated by first dissolving the mixture in heptane. Since “like dissolves like”‚ fluorene dissolves with the non-polar heptane and the polar fluorenone dissolves in the polar ethyl acetate solvent. This phenomenon was illustrated in class before the experiment‚ when it was pointed out why water will not dissolve fluorene‚ fluorenone‚ or transstilbene
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M&AE 305 October 3‚ 2006 Thin Airfoil Theory D. A. Caughey Sibley School of Mechanical & Aerospace Engineering Cornell University Ithaca‚ New York 14853-7501 These notes provide the background needed to implement a simple vortex-lattice numerical method to determine the properties of thin airfoils. This material is covered in Lecture‚ but is not in the textbook [5]. A summary of results from the analytical theory also is provided‚ as well as a comparison of the thin-airfoil results with those
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The Seven Layers of the OSI Model Kevin Ludwig Bryant and Stratton College TECH 140 Khaled Sabha 12.08.2009 There are seven layers in the OSI model. Each layer has a function or purpose that is used to set up a network. The International Organization for Standardization began to develop the OSI framework in 1984. The purpose was to have each layer interact with the layer directly beneath it. The layers are starting from the top: Application‚ Presentation‚ Session‚ Transport‚ Network
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