Fermentation of Carbohydrates: Ethanol from Sucrose Objective: To demonstrate a fermentation process‚ isolate the ethanol produced by fractional distillation‚ determine the composition of the ethanol solution recovered‚ and make stoichiometric and yield calculations. Procedures: Fermentation Weigh out 20.0 g of sucrose and place it into a 250-mL Erlenmeyer flask. Add 100 mL of water and gently shake until all the sucrose has dissolved. To this solution add 0.60 g of dipotassium hydrogen phosphate
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Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is usually
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Column Chromatography of Plant Pigments Jaybee Balilea‚ Sharmaine Baysic‚ Maria Anjelette Patricia Belen 3Bio-7‚ Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column Chromatography is a form of solid-liquid adsorption chromatography and depends on the essential principles as does in thin layer chromatography. It was used in this experiment in separating and analyzing the different components of Capsicum frutescens (siling labuyo) with the use
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EXPERIMENT 5: CHROMATOGRAPHY Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds‚ to compute Rf values and to identify unknown compounds using Rf values. The ten samples underwent paper chromatography to determine the components of the unknown sample. The mobile phase allowed
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What are Carbohydrates? Carbohydrates are the sugars‚ starches‚ and fibers found in fruits‚ grains‚ vegetables and milk products. Carbohydrates are sugars that break down inside the body to create glucose. Glucose is moved around the body in the blood and is the primary source of energy for the brain‚ muscles‚ and other essential cells. There are two types of carbohydrates‚ simple and complex; Simple carbohydrates refer to sugars with a simple molecular construction of one or two parts. Because
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Carbohydrate Loading There are many threats to an athlete during physical endurance competition whether it is a marathon or the decathlon. The most common forms of these threats include hypoglycaemia‚ fatigue‚ and loss of morale (which is usually caused by hypoglycaemia and fatigue). There is one connection that can be made between these threats and that is that all are caused one way or another through the lack of carbohydrates (Peterson). The most common way to combat these threats
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Community Outreach & Education Program CHROMATOGRAPHY (Adapted from: Forensic Science Activities. University of Colorado Boulder Hughes Initiative. .) DESCRIPTION: Students will use paper chromatography to separate ink molecules and identify the pen used on an unknown sample of handwriting. Students will graph and analyze data they collect using paper chromatography. PURPOSE/GOAL: Students will be able to: • Gain understanding of the purpose of chromatography. • Measure and graph pigment separation
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Column Chromatography of Plant Pigments Paul Ibarbia‚ Gene Paolo Jasmin‚ Gianpaolo Jimenez and Lorenzo Labicane* Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column chromatography of plant pigments is the separation of plant pigments extracted from Capsicum frutescens (siling labuyo). No slurry is prepared of the eluent; eluent with the stationary phase is set and then carefully poured into the column. The eluent are the solvents which are responsible for the
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How Chromatography Helps The Human Race Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. “Chromatography is done by making of a mixture move past the solids‚ or across the surface of a solid‚ like paper. The mixture is poured onto a solid surface. As the different components of the liquid run down the solid‚ some of them move more slowly than other. A component
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Carbon‚ Hydrogen and Oxygen. These three simple elements make up one of the most useful substances for many living organisms: Carbohydrates. Carbohydrates are used for numerous processes in organisms from acting in important roles of cellular recognition in the human body to storing chemical energy in plants in the form or starch. The roles of carbohydrates in the human body alone are numerous; providing energy for working muscles‚ providing energy for the regulation of blood glucose‚ preventing
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