As we know‚ the TLC is a separation technique that has two phases: the stationary phase and mobile phase. The stationary phase is silica gel (SiO2) which is very polar compound‚ while the mobile phase is 0.5% acetic acid which has less polar. Also‚ as we know‚ the compound will rather to stay with stationary phase or travel with mobile phase depend on its polarity. The result show as that the polarity of the solvent is the key factor for the compound to travel with it or stay in stationary phase
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Serratiopeptidase drug was purchased from MARS Therapeutics & Chemicals limited‚ Hyderabad‚ India. The standard drug Fenofibrate was purchased from USV limited‚ Himachal Pradesh‚ India. Assay kits for serum Total Cholesterol (TC)‚ Triglycerides (TGL)‚ High density lipoproteins were purchased from Erba Mannheim‚ Transasia Bio-Medicals limited‚ Himachal Pradesh‚ India. All other chemicals were of analytical grade. The experiment was conducted using [12] male Albino Wistar rats (150-200g)‚ at about
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The purpose of the lab “All in the Family” was to compare the reactivity of the halogens Chlorine‚ Bromine‚ and iodine by observing the reactions between their elemental forms and their ionic forms. To accomplish this experiment‚ we first added a squirt of pet ether to two test tubes. Then‚ we added the same amount of Cl2 to both of the tubes‚ shook the mixtures‚ and recorded the color that resided in each mixture as the initial color. After‚ a squirt of NaBr was added to one test tube‚ and a squirt
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Discussion: My results show the following: The unknown component traveled at a distance similar to the spice time blue dye. However‚ there are a few limitations within this experiment. The accuracy of the length of the component was difficult to record. The ruler that we used was degraded and thick‚ making it hard to read the length. The dyes could also cause error to occur. Everything has an expiration date‚ depending on how old or how new the dye is‚ it could affect the outcome. 1. Why is it
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Experiment: The intention of the experiment is to use solvent extraction methods to separate a mixture containing a carboxylic acid and a neutral compound. Once recovered‚ the solids were purified by recrystallization and examined by thin-layer chromatography‚ and their identities were derived by melting point and infrared (IR) spectroscopy. Extraction is a procedure that selectively dissolves one or more of the mixture compounds into a proper solvent. Extraction refers to the moving of compounds
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EXPERIMENT 15 Thin-Layer Chromatographic Analysis of Drug Components Experiment Objective: To identify the components of an analgesic drug tablet and then correctly identify the tablet from a group of others with acquired data. Experiment Summary: In this experiment‚ we use TLC to identify components of an unknown analgesic drug. We prepare a solution of the drug by dissolving part of a tablet in 1:1 ethanol/dichloromethane‚ then spotting a TLC plate with the solution along
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Chromatography (Greek for ‘colour writing’) is used to describe various methods applied to separate mixtures (referred to as the sample of the experiment) with great accuracy to analyze them. By using chromatography we can manipulate these to move at different speeds through the system‚ thus separating them. Chromatography is necessary in chemical industries‚ as well as bio processing companies. Chromatography can be: 1. analytical: used to measure ratios of analytes(substance in simpler forms)
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Mr. Sousa Organic Chemistry ACL 8 January 2015 Chromatography Chromatography is a physical method of separating substances based on their properties‚ by distributing their components between a mobile and stationary phase. Chromatography is useful for observing mixtures and solvents‚ since it can be used to determine the relative bond strength of various compounds‚ a substances phase‚ and it can also the identity of unknown substances. Chromatography allows for the separation of chemical mixtures
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provided for. Good luck!!! CHROMATOGRAPHY (By: Mayflor Markusic) KALIKASAN: Everyday Science in Action Volume 4 No. 1; pages 22-24 a) What is chromatography? 1 2 3 4 ____________________________________________________________ ________________ b) Give examples of mixtures which can be separated through chromatography. 4 5 6 7 ________________________________________ ______________________________________ c) How is chromatography done? 4 5 6 7 ________
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Chromatography is a process used to separate mixtures. The word chromatography is derived from the Greek words ‘khroma’ and ‘graphein’ meaning ‘color’ and ‘to write’ or to represent. Although there are a couple different types of chromatography‚ in each case a substance is placed onto or into a medium and a solvent is passed through the test substance. In chromatography science‚ the solvent is called the mobile phase or the carrier fluid and the medium is called the stationary phase. There are four
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