Stereochemistry of Dihydroxylation Reactions Question: Can the stereochemical outcome of dihydroxylation reactions be determined by thin layer chromatography? Overview: In this experiment‚ you will perform two reactions that transform alkenes into diols. While similar in functional group transformation‚ these reactions may lead to stereochemically different products. The two possible products are diastereomers of one another‚ and therefore have different physical chracteristics. After
Premium Thin layer chromatography Solvent Oxidizing agent
Abstract In order to experiment with distinct components of a mixture‚ they must be first separated so they can be observed individually. This is accomplished in this lab by the technique called thin layer chromatography (TLC). TLC involves a stationary phase‚ which the TLC plates as well as a mobile phase‚ which could be one of two solvents used: ethanol-acetone for TLC. Dyes in a sample separate consequently because of their unique polarities. As a result‚ nonpolar substances travel further
Premium Thin layer chromatography Chromatography
CODEINE AND PARACETAMOLPRACTICAL REPORT ON THE ISOLATION AND IDENTIFICATION OF CODEINE AND PARACETAMOL AIM: To extract codeine and paracetamol from its tablet by solvent extraction and tentatively identify in comparison to standards using Thin Layer Chromatography. INTRODUCTION: Codeine or methyl morphine‚ an alkaloid‚ was first isolated in 1832 from raw opium. It concentration ranges from 0.2% to 0.8%. Mostly used for its analgesic‚ anti-tussive and anti-diarrheal capabilities (Tremlett‚ Anderson
Premium Thin layer chromatography Chromatography
To: Wesley Clark From: Lizzie Heath Date: March 4‚ 2013 Subject: Thin-Layer Chromatographic Analysis of Drug Components (Experiment #15) The purpose of this experiment is to determine the components of an unknown drug and identify it as one of six (6) commercial drugs by using thin-layer chromatography. I added approximately a quarter of a tablet of acetaminophen‚ aspirin‚ caffeine‚ ibuprofen‚ salicylamide‚ and the unknown (#19) to separate test tubes containing 2.5mL of dichloromethane
Free Chromatography Thin layer chromatography Aspirin
pure crystal constituents of the compound. Thin layer chromatography can be used as a physical method to segregate compounds from natural sources. E.g. Spinach leaves are visibly green‚ but consist of a variety of components that have more colour than others. This experimental procedure uses compounds from spinach leaves that are exposed to chromatography‚ TLC plate to indicate the different pigments within the spinach extract. ‘Column chromatography in chemistry is a method used to purify individual
Premium Thin layer chromatography Chromatography Color
Chemical Separation Introduction: The objective of this experiment was to extract the pigments from spinach leaves‚ perform Thin Layer Chromatography (TLC) on the spinach leaf extract‚ and then determine the best solvent mixture to use to separate the pigments in the extract. The pigments are located inside the chloroplast walls in the cells of the spinach leaves. In order to obtain the pigments the cell walls must be broken down thus exposing the pigment containing chloroplasts. Upon
Premium Thin layer chromatography Purple Color
presence of 95% ethanol producing long red needles. 0.536g‚ 2.07mmol‚ 158-160°C (160°C lit3)‚ 54.6% recovery. Thin layer chromatography was performed in three different eluents‚ hexane‚ toluene‚ and acetone. The chromatography compared aniline‚ 2‚ 4-dinitrobromobenzene‚ and a sample from the mixture of the two reactants when the reaction first began to take place. Another thin layer chromatography used a product sample an hour after the reaction first began and the eluent that showed the best separation
Premium Thin layer chromatography Chromatography
IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different solubilities
Free Chromatography Thin layer chromatography
(Author’s name) (Professor’s name) (Course details) (Date Abstract The aim of the lab was to separate and analyse analgesic drugs in a drug tablet. The method used to separate the components was Thin Layer Chromatography (TLC) with silica adsorbent as the stationary phase and 0.5% glacial acetic as the mobile phase. In one plate‚ five known samples were used as the reference‚ that is: Aspirin; Caffeine; Ibuprofen; and Salicylamide. Aspirin and Salicylamide were the only samples that fluoresced
Premium Thin layer chromatography Chromatography Aspirin
nature of agarose‚ a solution of agarose can be heated and cooled to form a gel in a casting tray. Think of casting the agarose gel like pouring hot gelatin into a mold. The hot agarose liquid is poured into a casting tray. Once the mixture cools‚ a thin agarose brick will form. To ensure there’s a place to put the DNA in the gel‚ a comb is placed in the agarose liquid before it cools. Each tooth in the comb will become a hole‚ or ’well‚’ in the solidified agarose gel. Once cast‚ this gel is placed
Free Chromatography Thin layer chromatography Hydrochloric acid