Isolation of Ovalbumin and Enumeration of thiol groups Abstract Thiol groups are important to protein folding and forming disulphide bonds that are essential to protein structure. Determining the number of thiol groups in a protein is important in determining the tertiary structure of the protein. The ovalbumin is the experiment was purified from egg white using centrifugation and ammonium sulphate precipitation and then the thiol groups identified using DTNB and spectroscopy. The ovalbumin was found
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diet. Methionine has a thioether side chain‚ and cysteine’s contains a thiol group. These side chains exist as free thiols inside the cell‚ and are oxidised causing them to pair up and form disulphide bonds in an extracellular environment. Thiols are more reactive than hydroxyl groups and react easily with mercurails and heavy metal salts. The reaction with p-chloro-mercuribenzoate (PCMB) can be used to measure thiol groups‚ as there are changes in the ultraviolet spectrum of the compound. As proteins
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While researching how to cook meringue‚ I learned that meringue is created from the denaturation of ovalbumin‚ the main protein in egg whites. Denaturation is when a protein’s structure is disrupted. Ovalbumin is denatured by heat. In my science class‚ I had learned that heat and pH affect the denaturation of protein. I became curious about how changing the pH would affect the denaturation of ovalbumin when cooking meringue. So I decided to add lemon juice to egg whites and heat the egg whites. The
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of Comprehensive Research‚ Volume 5‚ Page 32 Groups and Group Behavior Dr. I. Chaneta Faculty of Commerce University of Zimbabwe ABSTRACT Groups and teams are a major feature of organizational life. The work organization and its sub-units are made of‚ are groups of people. Most activities of the organization require at least some degree of co-ordination through the operation of groups and teamwork. An understanding of the nature of groups is vital if the manager is to influence the behavior
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INDIVIDUALS A group can range in size from two members to thousands of members. Very small collectives‚ such as dyads (two members) and triads (three members) are groups‚ but so are very large collections of people‚ such as mobs‚ crowds‚ and congregations (Simmel‚ 1902). On average‚ however‚ most groups tend to be relatively small in size‚ ranging from two to seven members. One researcher ( J. James‚ 1953)‚ after counting the number of people in 7405 informal‚ spontaneously formed groups found in
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Groups can be defined by either informal groups‚ or formal groups. Formal groups A formal group is set by an official authority or leader designed to complete a specific task or purpose and is strictly regulated by the organisations rules and practices. In a formal group responsibility of an outcome of a task is set usually to the leader‚ discipline and reward is mainly handed down to the leader via the organisation. A formal group is coordinated by activities shared between a number of people
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influence others) Energetic (high activity level) Persistent Self-confident Tolerant of stress Willing to assume responsibility Clever (intelligent) Conceptually skilled Creative Diplomatic and tactful Fluent in speaking Knowledgeable about group task Organised (administrative ability) Persuasive Socially skilled McCall and Lombardo (1983) researched both success and failure identified four primary traits by which leaders could succeed or ’derail’: Emotional stability and composure:
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7/8/13 Outlook Print Message Re: Groups for IGD From: Class Rep (sechcr@gmail.com) You moved this message to its current location. Sent: 08 July 2013 20:09PM To: Abhinav Narayan (abhi15990@gmail.com); Abhishek Jay Kumar (abhijaykumar@hotmail.com); abhishek jha (abhishek.jha560@gmail.com); Adip Daniel (Adip.Daniel@gmail.com); Aditi Phadke (aditivphadke@gmail.com); agamagarwal1992@gmail.com; ajitha kondabala (ajitha.kondabala@gmail.com); akanksha.takyar@gmail.com; akash gupta (akash824@gmail
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1. What is Groupon and how does it work? What is Groupon’s market? What does this company do and how does it make money? Groupon is an online coupon that allows a customer who subscribes from Groupon.com to purchase or buy a definite service from a local business at a flat discount rate from 50 % to 90 %. A new Groupon is to be sent to the online subscribers each day and it has to be purchased before the specified time limit and it should have a minimum number if the buyers are decided before the
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INTRODUCTION Capital Mortgage Insurance Corporation (CMI) sells insurance to lenders protecting against mortgage default losses. They are a wholly owned subsidiary of Northwest Equipment Corporation. Following their acquisition in 1978‚ CMI’s goal has been to rebuild their business and diversify their services. Mortgage insurance is used to protect mortgage lenders (ie originators and/or underwriters) by transferring mortgage risk‚ and notably tail risk‚ from lenders to insurers. Insurers by their
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