the reagent in the 1940s. His 1951 paper describing the technique is the most-highly cited paper ever in the scientific literature‚ cited over 200‚000 times. The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is best used with protein concentrations of 0.01–1.0 mg/mL and is based on the reaction of Cu+‚ produced by the oxidation of peptide bonds‚ with Folin–Ciocalteu reagent (a
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In my ward‚ I have an experience of creating a track for buttonhole method which turned out to be successful. Buttonhole technique was initially used in Europe with people who had restricted cannulation sites. In this technique‚ the needle is implanted accurately at the same site and the same angle for every dialysis session. (Twardowski & Kubara‚ 1979). Formerly cannulation is mandatory with sharp needles to create a track formation. Ideally‚ the same person must cannulate the fistula to create
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Appendix A3: Study Methods for Student Surveys A3.1 Introduction We used two survey instruments to measure student outcomes from inquiry-based learning in undergraduate mathematics and to compare these outcomes between various student groups‚ in particular‚ between IBL and non-IBL students. The attitudinal survey was designed to detect the quality of and changes in students’ mathematical beliefs‚ affect‚ learning goals‚ and mathematical problem-solving strategies. The learning gains survey (SALG-M)
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Research Methods Abstract Every research study begins with a problem that researches want to solve. For every problem to be researchable it must be the one that can be studied by collecting data and analyzing it. The steps without which the data collected will not move on into research process are: stating the problem‚ designing the research question and developing a hypothesis and/or purpose statements that will be supported at the end of the study. The first thing a researcher needs to
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Scientific Method The Scientific Method is a system devised to allow scientists to gain insight‚ or knowledge‚ on a wide range of questions about one’s behavior and other points of interest. The first step in this process is to identify a question that deserves an explanation or answer to. For example‚ a good indentifying question that one could use would be why did the chicken cross the road? This is a specific behavior that can be addressed and quantified. Once the individual has the question
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Introduction In order to identify new materials‚ scientists use a variety of chemical and physical methods to determine molecular masses. One of these methods includes the Dumas method for determining the molecular weight of a volatile liquid. This method‚ which was proposed by John Dumas in 1826‚ makes use of a volatile liquid (vaporizes at a relatively low temperature) and allows this liquid to be heated in a water bath to a known temperature and escape from a flask through a tiny opening (Giunta
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questionnaire the guess‚ age‚ and gender facets. 3) Choose a reasonable sample size of people and ask them to fill out the ballots. 4) Recollect the questionnaires and enter the data into Excel. 5) Sample the guesses into 5 groups utilizing the bootstrapping method found within statistics. 6) Plot the results of the bootstrapped data and the data obtained from the other facets from the questionnaires. Draw conclusions. In Step 1‚ put an undetermined amount of jellybeans in the jar to ensure you didn’t know
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Inquiry Method Steps 1. Ask a question or identify the problem – What do you want to learn about? This question forms the starting point for inquiry. Example: You may be interested in discovering what methods of studying lead to better exam results. 2. Develop a hypothesis – A hypothesis is possible answer to your research question. Your hypothesis gives you direction. It helps you to determine what needs to be tested and which research method you will use. Example: You may hypothesize that
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(DPPH) for estimating antioxidant activity Songklanakarin J. Sci. Technol.‚ 2004‚ 26(2) : 211-219 The use of the stable free radical diphenylpicrylhydrazyl (DPPH) to estimate the activity of antioxidants is reviewed. Current applications of the method are examined‚ particularly the use of the parameter EC50 (substrate concentration to produce 50% reduction of the DPPH). Some recommendations are made as to the most suitable ways of carrying out this assay and evaluating the data produced. Key
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ICT Methods for Communication “Information and communication technologies are defined as for the purposes‚ as a “diverse set of technological tools and resources used to communicate‚ and to create‚ disseminate‚ store‚ and manage information.” These technologies include computers‚ the Internet‚ broad casting technologies (radio and television)‚ and telephones”. (Mohanty 2011) The use of ICTs in today’s society has rapidly increased through the use of social media sites such as Facebook‚ twitter
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