EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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L2 Certificate in the principles of business admin Unit 201 - Principles of personal responsibilities and working in a business environment 1.1 – Identify the main points of contracts of employment When starting a new job everyone will be given a contract of employment by the employer‚ in this contract there are a few points that you will notice such as your name‚ employers name‚ where you will be working‚ contact information etc. The most important points you will find are * Salary – This is
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Introduction Molecular materials with quadratic nonlinear optical (NLO) properties are currently attracting considerable interest [1–8]. The quadratic hyperpolarizability (b) of a chromophore is associated with charge transfers between donor and acceptor substituents linked through p-conjugated pathways. Most NLO
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CHAPTER THREE RESEARCH DESIGN AND METHODOLOGY RESEARCH DESIGN This study entitled "The Effects of Technology on Discipline in the Pre-school Montessori Classroom" is a qualitative research that attempts to accumulate existing information and data regarding the governing principles and systems of Montessori education in the modern times. Trochim stated that "a key reason for doing qualitative research is to investigate and become more experienced with a particular phenomenon
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v Describe what Montessori meant by’ New Education’ Dr. Maria Montessori is the creator for the Montessori Education Method for a new world who devoted her life to improve children’s education excellence. Her educational method is widely used in schools or at home for children 3 t0 6 years old. Maria Montessori lived through one of the traumatic time eras of the world history‚ which changed everybody’s lives including children. It was the time of anxiety‚ cruelty‚ death‚ family separation and
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must be included employer’s and employee’s expectations from each other: duties and responsibilities‚ hours of work‚ salary‚ holiday entitlement. 2a) List three key points of legislation that affect employers in a business environment. Health and safety‚ pay and pensions‚ employment rights and responsibilities 2b) List three key points of legislation that affect employees in a business environment. Health and safety‚ pay and pensions‚ employment rights and responsibilities
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Wood/timber Building material these are raw woods that have been converted in the forms of minimally- processed logs stacked on each other‚ timber frame construction‚ and light- frame construction. It is used as load- bearing material depend on the quality criteria. Concrete composite building made from combination of aggregate material and a binder such as cement. It is one of the most durable building materials. It provides superior
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Unit one: Principles of personal responsibilities and working in a business environment Assessment You should use this file to complete your Assessment. • The first thing you need to do is save a copy of this document‚ either onto your computer or a disk • Then work through your Assessment‚ remembering to save your work regularly • When you’ve finished‚ print out a copy to keep for reference • Then‚ go to www.vision2learn.com and send your completed Assessment to your tutor via your My
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Clarification for Shaft Material of Condensate Extraction Pump: Subject: Shaft Material Clarification FEED Requirement: Cl. no. 6.5.7.4 "The shaft‚ impellers‚ stationary and rotating wear rings shall be stainless steel. The casing shall be carbon steel. Materials shall comply with API 610 Appendix H‚ material class S-8." Explanation: SDC contacted to all reputed suppliers for Condensate pump in Korea & Japan. They have majorly suggested the below mentioned material of construction which
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The Scarlet Letter‚ Nathaniel Hawthorne emphasizes the intricate themes of isolation and alienation. Using a variety of literary techniques and descriptions of emotions and nature‚ Hawthorne is able to fully depict the inner feelings of hurt suffered by the central characters as a result of severe loneliness and seclusion. This‚ therefore‚ further adds to the overall gloomy and cynical atmosphere of the work. Isolation and alienation‚ two forms of torturous estrangement‚ are experienced by the key
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