Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state. When protein is bound to the dye the color turns blue. In this experiment a standard was used to start the experiment‚ specifically the BSA stock solution. This standard served as the “control” group of the experiment. Due to the
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Methods and Materials In this experiment‚ three dye molecules were used to calculate the solubility of the chemical structures in the relation to Rf . The Carmine dye was used because it contain six –OH bonds. Since‚ six –OH bonds were identified in this molecule the Rf results obtained‚ should differ greatly from the results taken from the other molecules. Alizarin (red) dye was chosen because it has one -OH bond. The third molecule Azure chosen because it does
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(cm) D2(cm) Calculated Rf Component Rf (from table 2) Green Colouring Yellow‚ Blue B: 8.15 Y: 6.55 8.15 B: 1.0 Y: 0.80 B: 1.0 Y: 0.89 Unknown Mixture Red‚ Yellow‚ Blue R: 5.72 Y: 6.18 B: 7.10 7.10 R: 0.81 Y: 0.87 B: 1.0 R: 0.78 Y: 0.89 B: 1.0 Table 4 Dye Red #2 Red#3 Red#4 Yellow#5 Yellow#8 Blue #1 Blue #2 Rf 0.81 0.41 0.62 0.95 0.77 1.0 0.79 Observations The solvent (water) always travelled farther than the solute‚ except in the case of blue‚ which travelled the same amount as the solute. The blue
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exciting solutions happening throughout the life cycle of this pioneering industry. Impacts and Concerns The environmental footprint of creating apparel varies based on the methods used to produce and extract the resources; the process to create‚ dye and finish the materials; the steps to cut‚ sew and assemble the products‚ and the packaging and distribution systems to move and deliver the product. Examples of the environmental impacts of these steps are discussed below: Conventional Cotton Farming
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The U.S Constitution was written in 1787. The Founding Fathers “recognized that government depended on the consent of the governed” (Dye & Gaddie‚ 80). The citizens of the United States give the government permission to have a government. The Founding Fathers are very significant because they provided limits on the power of the majority and safeguarded the rights of the minority. The Bill of Rights also provides safety for the minority and does not allow the majority to exceed their power. The paradox
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the experiment‚ the stationary phase was the filter paper onto which the dye samples were dropped onto while the mobile phase was the solvent mixture containing ethanol and water which carried the sample dyes as the mixture went up the paper at different rates. These rates depend on the solubility and polarity of compounds in solvent. The objectives of this experiment are to compute the retention factor values of 6 sample dyes: methyl
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hair coloring history came in 1863‚ when chemist Dr. August Wilhelm Von Hofmann reported the dye properties of paraphenylenediamine. His discovery led to the birth of the synthetic hair dye industry‚ and PPD still Dominates the field today. Hofmann was also known for his studies of organic derivatives of ammonia and for discovering the first unsaturated alcohol and several organic dyes. On the heels of Hofmann’s discovery‚ in 1867 London chemist E. H. Thullay and Parisian hairdresser
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CHAPTER 1 Introduction Background of the Study Nowadays‚ most documents need to be printed. For most students and office workers‚ their homes are not complete without the printer. Owning this device is really just a necessary convenience in their everyday lives. However‚ despite the very high demand for printers‚ inks and paper in the modern world‚ it is overlooked that these materials are expensive to make. Printers and printer inks cost several hundred pesos and few people have the privilege
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identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation that does stain biological samples. Examples of acidic dyes include acid fuchsin and eosin; examples of basic dyes include crystal violet‚ methylene blue and safranin. As a general rule‚ basic dyes are attracted to the surface of
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Chromatography of Food Dyes Name: Cassandra DeVaux Date: April 17‚ 2014 Purpose: To learn how mixtures of compounds can be separated and what food dyes are found in certain foods Introduction: In this exercise we will separate food dyes from a variety of sources. We will first gather data on known dyes where we know how many substances make up the dye. Then we will collect data on some samples where we do not know the make-up. We will compare our known dyes with our unknown samples
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