DESIGN 9 2. Nucleic acid purification 9 3. Reverse transcription 9 4. Controls and normalization 9 5. Standard curve evaluation of efficiency‚ sensitivity‚ and reproducibility 9 Real-Time PCR Fluorescence Detection Systems 12 DNA-Binding Dyes 12 Primer-Based Detection Systems 13 PROBE-BASED DETECTION SYSTEMS 14 Hybridization probes (also called FRET probes) 16 MELTING CURVE ANALYSIS 16 Multiplex real-time PCR 18 APPLICATIONS OF REAL TIME PCR 18 GENE EXPRESSION ANALYSIS 18
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(Master budget preparation) Sopchoppy Company manufactures a red industrial dye. The company is preparing its 2000 master budget and has presented you with the following information. 1. The December 31‚ 1999‚ balance sheet for the company is shown below. SOPCHOPPY COMPANY Balance Sheet December 31‚ 1999 Assets Liabilities and Stockholders’ Equity Cash $ 5‚080 Notes Payable $ 25‚000 Accounts Receivable 26‚500 Accounts Payable 2‚148 Raw Materials Inventory
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everything the client likes to express. Also‚ the drawing materials will be provided by the instructor. The title of the activity is in relation with nature‚ so as to help preserve Mother Earth‚ the instructor will give materials that are natural and dyes which are from the environment. Various coloring materials are extracted from some of the world’s natural resources‚ in such way‚ both the mentally ill clients will be able to help conserve the environment‚ and be able to express themselves as well
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results of the equation shows sound financial position of the company. Page | 3 Introduction: Berger is one of the oldest names in the paint industry. The name came from a German national‚ Louis Berger who founded dye and pigments making business in England. Production of dyes and pigments evolved into productions of paints and coatings. The company grew rapidly by establishing
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Apparatus: -Osborne Reynolds apparatus. -Hydraulics Bench. -measuring cylinder. -Stopwatch. -Vegetable Dye. -Thermometer. 4. Procedure: * Fill the reservoir with dye‚ position the apparatus on the bench and connect the inlet pipe to the bench feed. Lower the dye injector until it is just above the bell moth inlet. Close the control valve. Open bench inlet valve and slowly fill the head tank to the overflow level
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separate different-sized molecules in a porous‚ sponge-like matrix. 2. What is the purpose of the agarose gel? It is used to separate DNA molecules that range in different lengths. 3. What is the purpose of adding blue “tracking” dye to the DNA samples? The blue tracking dye is added to help load the samples easily and helps able to see the DNA moving through the gel. 4. Explain why DNA has an overall negative charge. The phosphate groups in the DNA backbone carry negatively-charged oxygens‚ which
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have to take yellow and blue to make that green. Plus the pigments that were shown on the paper. (5 points) 6. You are given an unknown type of clothing dye. How could you use the procedures in this lab to see if this dye is a mixture? Answer: In order to find out if the clothing dye is a mixture‚ you would rub a small amount of the dye on the filter paper just like you would the dot of ink. Then place it in your beaker or some container of
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membrane structure Introduction Beetroot Pigments Beetroots contain Betalains which are the red pigments present in the cell vacuole. Betalains are soluble in water and they contain nitrogen. Betalains extracted from beetroot is commonly used as food dye because it is not known to cause any allergic reactions. Beetroot Picture taken from http://tipdeck/how-to-cook-beet-root Structure of Betalain Picture taken from http://en.wikipedia.org/wiki/File:Betanin.png Cell Membrane Cell membrane
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define Beer’s law and define the relationship between absorbance and transmittance. Other learning objectives are to create a Beer’s law plot for a series of samples with known concentrations‚ collect spectrophotomic data from unknown and known FDC blue dye samples‚ perform serial dilutions‚ calculate concentrations‚ perform linear regression and determine the equation of a best fit line. DISCUSSION/OBSERVATION A solution is composed of a solute dissolved into a solvent. The most common solvent is water
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Investigating the effect of pH on the activity of the enzyme catalase. Introduction Hydrogen peroxide (H2O2) is a very pale blue liquid which appears colourless in a dilute solution‚ slightly more viscous than water. It is a weak acid. It has strong oxidizing properties and is therefore a powerful bleaching agent that is mostly used for bleaching paper. Catalase is a common enzyme found in all living organisms. Its functions include the conversion of Hydrogen Peroxide‚ a powerful and potentially
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