Catalase (Enzymes) Abstract In this laboratory exercise‚ studies of enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the
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The Behavior of Proteins: Enzymes Enzymes are Effective Biological Catalyst Catalysis- speeds up metabolism to allow production of products. Enzymes- Highly specific and most efficient catalyst that speeds up metabolism or rate of reaction in organisms by factor up to 10^20 (globular proteins) Nonenzymatic catalyst- enhance by 10^2 -10^4 Ribozymes- acts for catalytic activity in RNA’s Kinetics versus Thermodynamics Standard free energy change- difference between the energies of the reactants
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Enzyme Lab: Peter Kuetzing – 10/4/2012 – F Block 1. How does changing the concentration of enzyme affect the rate of decomposition of H2O2? When more enzymes is added the rate of reaction speeds up. In this case‚ H2O2 will produce more O2‚ in return the kpa/min will go up. 2. What do you think will happen to the rate of reaction if the concentration of enzyme is increased to five drops? Predict what the rate would be for 5 drops. I think that the rate of reaction will slightly increase from
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Introductory Biology 1 Biology 1003 Fall Term 2011 Lab Number: 3 Title: Cell Energetics: Enzyme Role in Biological Reactions Name: Brandon Moore Student Number: 100819124 Lab day and time: Wednesday pm Date: Wednesday November 23‚ 2011 Introduction Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology
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Property of Starch The experiment obtained Starch to have a bulk density of 0.48g/ml‚ i.e. 0.48g of Starch occupies 1ml of water. This value represented the density of starch incorporating the voids. The tapped density was found to be 0.60g/ml. This value represented the density of starch excluding the voids and intra- particle pores greater than molecular and atomic dimensions in the crystal lattice. This simple means‚ by reducing the number of intermolecular spaces between the starch particles‚
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Experimental Analysis on Enzymatic Behavior of Human and Fungal Amylase Lab name and number: Enzymes‚ Lab #5 Panther I.D: 2640403 Shayra Medal Instructor: Emily Nodine Section U21 October 26‚ 2011 X_______________________ Abstract Section The concept of this experiment was to analyze the enzyme Amylase and its environmental behavior. Amylase breaks down the biological macromolecule‚ carbohydrates‚ specifically starch into condensed subunits categorized as monosaccharaides or disaccharides
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Enzymes control the rate of metabolic reactions‚ they act as biological catalysts‚ which means they are used but not used up and they also control the speed of the reaction. Enzymes are proteins which means that anything that disrupts this structure such as high temperature or change in pH will affect the enzyme activity. There are many factors affecting enzyme action for example temperature effects them‚ if the Increasing the heat gives molecules more kinetic energy so they vibrate this can then
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IODIMETRIC AND IODOMETRIC METHOD SUBSTANCE TO BE ASSAY AQUEOUS/NON-AQUEOUS ALKALINITY / ACIDIMETRY DIRECT/RESIDUAL TITRATION TITRANT INDICATOR CHEMICAL REACTION Assay of Antimony potassium tartrate Direct titration 0.1 N Iodine Starch TS KOOCCHOHCHOHCOO (SbO) + I2 + H2O KOOCCHOHCHOHCOO (SbO2) + 2HI + 2HI + 2NaHCO3 2NaI + 2H2O +
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sizes of molecules and how the chemical reactions take place. Therefore‚ the experiment was conducted using glucose and starch solution inside the dialysis tube. The starch and glucose that was put inside the dialysis tube help identify which of the two will reacted with potassium iodide inside the breaker‚ as the latter passed from the beaker into the tube‚ the glucose/starch solution’s change of color showed that the potassium iodide was small enough that it able to pass through from the solution
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There are many reasons why enzymes have such a high specificity. The first variable is an enzyme’s primary structure. A primary structure is just a combination of amino acids. There are twenty different amino acids that the primary structure can be created from. Every enzyme has a different order that the acids are placed in and each one has a different number or amino acids. The slightest change in this structure can affect a protein’s conformation and function. The secondary structure is a regular
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