Layer Chromatography (TLC) 1. Obtain 2 silica-gel-coated TLC sheets and 1 alumina coated TLC sheet. Measure up 1 cm from the bottom of the TLC strip. Taking care not to damage the white‚ powdery stationary phase‚ use a #2 pencil to draw a line across the width of the strip. Carefully mark two evenly spaced spots along the line to delineate where to spot the dye solution. 2. The TLC strip can now be spotted. Use the capillary tube to apply two dots of the dye onto the TLC strip as in the figure
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Task 3: How could Thin Layer Chromatography (TLC) have been used to prepare a pure sample of aspirin? The method of using thin layer chromatography is easy‚ quick and relatively cheap to carry out this makes it ideal for preparing pure samples of aspirin. Equipment Required: • Thin Layer Chromatography Paper • Ruler • Pencil • Solvent • Beaker • Micro Capillary • Access to a fume cupboard • UV light • Clingfilm • Individuals sample of aspirin • Pure sample of aspirin • Sample of salicylic acid Method:
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SWOT Analysis I selected the business plan for a wedding consultant business‚ TLC Wedding Consultants. Utilizing the SWOT method for analysis‚ I reviewed and analyzed the business plan for strengths‚ weaknesses‚ opportunities‚ and threats. Strengths and weaknesses analyze internal factors of the business. Opportunities and threats analyze external factors of the business. TLC Wedding Consultant’s business plan was well organized and clearly shows the business can be viable and strong. The
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method with 70% ethanol. Total antioxidants activities of phenolic and flavonoid content have been determined using Folin-Ciocalteau reagent and aluminium chloride colorimetric assay‚ respectively. Then the extracts were evaluated for their antioxidants activities by using DPPH free radical scavenging assay. From the results obtained‚ sky fruit kernel showed higher phenolic content (17.44 g GAE/100g dry extract) and flavonoid content (189.43 g QE/100g dry extract) than sky fruit seed coat (2.70 g
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purify complex mixtures of compounds. In this experiment‚ TLC is used to determine the best solvent system for the column. Due to Flash-Column Chromatography1 being an inexpensive technique‚ many scientist use this process for drug and natural product purification. Method By using the information from Experiment 6B‚ the TLC solvent system used was 30% EtOAc: 70% Hex. Experiment 7 was broken into two parts‚ Part One and Part Two. For Part One TLC was used to determine the eluents and the polarity of
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TLC is used because it is “sensitive‚ fast‚ simple and inexpensive analytical technique”2 (Mohrig 151). The unknown solid (unknown #3 in this case) used in the experiment was obtained and added to a 2.5ml of Ethanol/dichloromethane in a test tube. This was the solvent used in the experiment because Dichloromethane volatility and ability to dissolve a wide range of organic compounds makes it a useful solvent for many chemical processes. The solid was already in powder form therefore we did not have
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Chromatography is to separate the mixture of individual compounds from the spinach leaf sample used. Material and apparatus: Part 1 : Analysis of Analgesic Drugs Material: Aspirin Acetaminophen Caffeine Unknown A Unknown B TLC plates Ethyl acetate Hexane Acetic acid Iodine Apparatus: UV Lamp Capillary tube 250mL beaker Filter paper
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I’d first like to say that while errors did occur during your TLC you did a good job getting visible results on your TLC plate‚ as sometimes it can be hard to get a sample to show up clearly on the plate. But not knowing which compound is which is a huge problem that will lead to incomplete conclusions and the need to redo your TLC. However‚ due to the knowledge we have of the compound on the plate we can determine which compounds are which. The two compounds you used are very similar in structure
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Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli Proponents: John Rovic P. Ngo Lester T. Almarez Trisha Mae N. Reyes August 2013 In Partial Fullfillment of the Requirements in Research II-B Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli A Science Research Project Presented to The Faculty of Science and Technology Mr.June Hayden Sinson Research Adviser
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Thin Layer Chromatography Introduction Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography‚ but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two phases
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