overdose‚ people also overdose on common over-the-counter (OTC) drugs‚ like aspirin‚ when attempting to take their own lives. Thin-layer chromatography (TLC) is one technique used to identify unknown drugs. Chromatography is simple to perform‚ is straightforward to interpret‚ and works equally well for legal and illegal substances. The experiment uses TLC to identify the active ingredients in some common OTC painkillers. Introduction: The majority of evidence submitted
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the separation. Thin layer chromatography (TLC) is a chromatographic technique that we use to separate the components of a mixture using a thin stationary phase such as silica plate supported by an inert backing. It applies mainly in medical‚ biological‚ chemical and pharmaceutical sciences because it is easy to be done and saves time because the reaction is fast. This method used for identifying mixtures by separating the compounds in the mixture. TLC is also used to help determining the number
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recorded in lab. However‚ spot results were recorded. The TLC plates 1-3 showed the presence of starting material (SM) in the crude product (CP) lane. TLC plate 1 was taken after adding the initial addition of 0.25mL of bleach. TLC plate 2 was taken after adding an additional 0.08mL of bleach because plate 1 still showed presence of SM in the CP lane. TLC plate 3 was taken after adding an additional 1mL of bleach for the same reason. However‚ TLC plate 4 did not show any SM in the CP lane and this was
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ABSTRACT Scientists continue to search for and conserve little-known plants those whose medicinal properties have become crucial in the fight against diseases. Moreover‚ lessons from folk medicine‚ indigenous knowledge and Chinese medicine on crude extracts points to possible finding of novel promising and strong pharmaceutically bioactive constituents. Arum palaestinum‚ commonly known as Black Calla Lily‚ is one of the important medicinal plants belonging to family Araceae which has not been well
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ISOLATION OF TRIMYRISTIN FROM NUTMEG ABSTRACT The purpose if the experiment was to extract the Trimyristan from ground nutmeg using extraction‚ distillation and recrystallization procedures. This experiment involves extraction and separation techniques that yield a purified product. The product was extracted and a distillation process was used to remove any remaining solvent. The recrystallization of the sample in turn produced a pure sample‚ which was proven using the known melting
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conophorum leaves contains saponins‚ tannin‚ steroid‚ flavonoid‚ terpenoids and cardiac glycosides as their major secondary metabolites while alkaloid‚ phlobatannin and anthraquninone were absent (Table 4). This result was contrary to the report of Ayoolaet al.[33] who reported presence of alkaloid‚ tannin and absence of saponins‚ steroid‚ flavonoid‚ terpenoids‚ cardiac glycosides‚ phlobatannin and anthraquninone for T. conophorum seeds while some of
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different kinds of functional groups and number of functional groups of molecules will determine the separation. Depending on what kind of solute‚ solvent‚ and adsorbent used‚ molecules will experience the interaction during dynamic equilibrium. On a TLC plate‚ capillary tube is used to transfer o- and p- hydroxyacetopheone‚ taking advantage of capillary force to make small spotting. A 30:70 ether acetate: petroleum ether is used as a solvent‚ which will not interact with surface of the silica gel due
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Prepare a TLC chamber by cutting a large circle of filter paper in half and placing it in a beaker. Add ~ 6 mL of methylene chloride (or 30% acetone in heptanes-needs to be fresh‚ otherwise acetone will evaporate) and cover the beaker with a watchglass. For each TLC run‚ you will need a silica gel plate and two clean capillary tubes for spotting the plate. Draw a horizontal line near the bottom of each TLC plate (the line must be higher than the level of solvent in the TLC chamber). Draw three
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for treating various kinds of diseases. In this work‚ 10 genotypes of T. foenum-graceum were evaluated for their diosgenin content and productivity‚ ascorbic acid (AA)‚ total phenolics (TP)‚ flavonoids (TF)‚ tannin and antioxidant activity (AOX). Results indicate that fenugreek is a good source of flavonoid and total phenolics ranging from 2.74to 12.03 mg/g and 22.59 to 62.59 mg/g‚ respectively. Diosgenin content ranged from‚ 6.12 to 10.98 mg/g respectively. One way ANOVA analysis was performed to
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The total protein content was estimated as proposed by Lowry et al. A bovine serum albumin stock solution (1mg/ml) was prepared in sodium hydroxide (1N). Five different concentrations (0.2‚ 0.4‚ 0.6‚ 0.8‚ 1 ml) of the prepared solution were taken in different test tubes. In another set of test tubes‚ 0.1 and 0.2 ml of the extract were taken. In each test tube‚ the volume was made up to 1 ml‚ followed by addition of the prepared alkaline solution (5 ml) at room temperature. The solutions were left
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