analyze the components of the spinach extract. This was done using a TLC plate and spotting each of the components on to the plate as well as spotting the standard spinach extract. A beaker was used with the developing solvent‚ filter paper‚ and TLC plate inside and a watch glass over top of the beaker to perform the thin layer chromatography. After the solvent front (30% ethyl acetate in hexane) reached about an inch from the top‚ the TLC plate was removed from the beaker and the developing spots were
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Cognitive Decline. What is cognitive decline: Most people do not know this term. Cognition refers to your ability to think so cognitive decline is a decrease in your ability to think. This includes all mental functions‚ including memory‚ calculation the ability to speak‚ abstraction and judgment. The actual frequency of this problem is unknown. Large numbers of affected people go undetected. People use the term dementia is often used to mean the same thing‚ but normally implies a much more dramatic
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aspirin collected. d. Weight of unknown compound isolated e. Weight of recrystallized unknown compound f. Melting point range of recrystallized unknown compound. g. TLC sketches with appropriate data for Rf calculations (Clearly label each column of spots on the page so I know what it is) h. Mixed melting point based on information from TLC Final Report
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segregate compounds from natural sources. E.g. Spinach leaves are visibly green‚ but consist of a variety of components that have more colour than others. This experimental procedure uses compounds from spinach leaves that are exposed to chromatography‚ TLC plate to indicate the different pigments within the spinach extract. ‘Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds’ (Wikipedia‚ 2011). This method is similar to thin layer
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experiment aims to make the students capable of separating the colored components of siling labuyo and malunggay leaves using column chromatography. Determine the purity of the components using the thin layer chromatography (TLC). Measure the Rf values of the colored components in TLC. Chromatography is the most orient laboratory technique that deals with separation of homogenous and free of interferences mixtures and identification of compounds. Also‚ it is essential for analyzing complex compounds
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stationary phase” (Brozek). Thin Layer Chromatography (TLC) is a solid-liquid technique in which the two different phases are a solid stationary phase and a liquid mobile phase. TLC can be used to detect a given compound in a mixture‚ follow the course of the reaction‚ follow the purification of a compound‚ or evaluate the purity of the compound. Objectives: Certain objectives needed to be met in order for the Thin Layer Chromatography (TLC) experiment to be understood and completed. The identification
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Experiment 1: Mechanism of Nitrile Oxide and Alkene Cycloaddition 1. Purpose: to clarify the mechanism for the cycloaddition reaction between benzonitrile oxide and an alkene‚ and to test the regiochemistry of the reaction between benzonitrile oxide and styrene. 2. Plan: Each student in a group of three will work to create a reaction with the Benzonitrile Oxide with‚ cis-stilbene‚ trans-stilbene‚ or styrene in an Erlenmyer flask. With this Reaction solution thin layer chromatography will be
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and a pure salicylic acid has 158 °C. TLC is used to separate compounds based on affinity for stationary phase or mobile phase. In TLC‚ the solvent went up at different rates. The retention factor (Rf) of a component can then be measured by dividing the distance that was traveled by the solvent front distance.By doing this experiment‚ the number of moles and grams of salicylic acid and acetic anhydride can be answered. Methods/ Material: Capillary tube TLC Plates Gloves 250 mL‚ 600 mL beakers Salicylic
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Solid October 6‚ 2013 Introduction: This experiment was focused on the cooperative identification of organic compound by its chemical properties such as: slow melting point‚ mixed melting point‚ Rf values in TLC experiment‚ IR spectrum analysis‚ and H NMR spectra. Such data can provide the the identity of functional groups and the identity of the compound itself. In this experiment students were taking the slow melting point of their unknown compound. The
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The reactant is a secondary alcohol therefore there would be no over-oxidization. The purity of the benzophenone by recrystallization will be analyzed using thin layer chromatography (TLC) analysis. Two TLCs is done in this experiment. The first TLC plate is just to monitor the progress of the reaction. The second TLC is to monitor the progress of the recrystallization. In the second part of the experiment‚ the goal is to determine which test tubes with unknown A and B contains glucose or sucrose.
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